Abstract
We report the observation of chromatin dynamics in living budding yeast (Saccharomyces cerevisiae) cells, in three-dimensions (3D). Using dual color localization microscopy and employing a Tetrapod point spread function, we analyze the spatio-temporal dynamics of two fluorescently labeled DNA loci surrounding the GAL locus. From the measured trajectories, we obtain different dynamical characteristics in terms of inter-loci distance and temporal variance; when the GAL locus is activated, the 3D inter-loci distance and temporal variance increase compared to the inactive state. These changes are visible in spite of the large thermally- and biologically-driven heterogeneity in the relative motion of the two loci. Our observations are consistent with current euchromatin vs. heterochromatin models.
Original language | English |
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Article number | #304344 |
Pages (from-to) | 5735-5748 |
Number of pages | 14 |
Journal | Biomedical Optics Express |
Volume | 8 |
Issue number | 12 |
DOIs | |
State | Published - 1 Dec 2017 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Atomic and Molecular Physics, and Optics