Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

Rotem Ben-Tov Perry; Ida Rishal; Ella Doron-Mandel; Ashley L Kalinski; Katalin F Medzihradszky; Marco Terenzio; Stefanie Alber; Sandip Koley; Albina Lin; Meir Rozenbaum; Dmitry Yudin; Pabitra K Sahoo; Cynthia Gomes; Vera Shinder; Wasim Geraisy; Eric A Huebner; Clifford J Woolf; Avraham Yaron; Alma L Burlingame; Jeffery L Twiss; Mike Fainzilber

doi:10.1016/j.celrep.2016.07.005

Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

Rotem Ben-Tov Perry, Ida Rishal, Ella Doron-Mandel, Ashley L Kalinski, Katalin F Medzihradszky, Marco Terenzio, Stefanie Alber, Sandip Koley, Albina Lin, Meir Rozenbaum, Dmitry Yudin, Pabitra K Sahoo, Cynthia Gomes, Vera Shinder, Wasim Geraisy, Eric A Huebner, Clifford J Woolf, Avraham Yaron, Alma L Burlingame, Jeffery L TwissMike Fainzilber

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin beta1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin beta1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin beta1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.

Original language	English
Pages (from-to)	1664-1676
Number of pages	13
Journal	Cell Reports
Volume	16
Issue number	6
DOIs	https://doi.org/10.1016/j.celrep.2016.07.005
State	Published - 9 Aug 2016

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Perry, R. B.-T., Rishal, I., Doron-Mandel, E., Kalinski, A. L., Medzihradszky, K. F., Terenzio, M., Alber, S., Koley, S., Lin, A., Rozenbaum, M., Yudin, D., Sahoo, P. K., Gomes, C., Shinder, V., Geraisy, W., Huebner, E. A., Woolf, C. J., Yaron, A., Burlingame, A. L., ... Fainzilber, M. (2016). Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size. Cell Reports, 16(6), 1664-1676. https://doi.org/10.1016/j.celrep.2016.07.005

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title = "Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size",

abstract = "How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin beta1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin beta1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin beta1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.",

author = "Perry, \{Rotem Ben-Tov\} and Ida Rishal and Ella Doron-Mandel and Kalinski, \{Ashley L\} and Medzihradszky, \{Katalin F\} and Marco Terenzio and Stefanie Alber and Sandip Koley and Albina Lin and Meir Rozenbaum and Dmitry Yudin and Sahoo, \{Pabitra K\} and Cynthia Gomes and Vera Shinder and Wasim Geraisy and Huebner, \{Eric A\} and Woolf, \{Clifford J\} and Avraham Yaron and Burlingame, \{Alma L\} and Twiss, \{Jeffery L\} and Mike Fainzilber",

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year = "2016",

month = aug,

day = "9",

doi = "10.1016/j.celrep.2016.07.005",

language = "الإنجليزيّة",

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Perry, RB-T, Rishal, I, Doron-Mandel, E, Kalinski, AL, Medzihradszky, KF, Terenzio, M, Alber, S, Koley, S, Lin, A, Rozenbaum, M, Yudin, D, Sahoo, PK, Gomes, C, Shinder, V, Geraisy, W, Huebner, EA, Woolf, CJ, Yaron, A, Burlingame, AL, Twiss, JL & Fainzilber, M 2016, 'Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size', Cell Reports, vol. 16, no. 6, pp. 1664-1676. https://doi.org/10.1016/j.celrep.2016.07.005

TY - JOUR

T1 - Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

AU - Perry, Rotem Ben-Tov

AU - Rishal, Ida

AU - Doron-Mandel, Ella

AU - Kalinski, Ashley L

AU - Medzihradszky, Katalin F

AU - Terenzio, Marco

AU - Alber, Stefanie

AU - Koley, Sandip

AU - Lin, Albina

AU - Rozenbaum, Meir

AU - Yudin, Dmitry

AU - Sahoo, Pabitra K

AU - Gomes, Cynthia

AU - Shinder, Vera

AU - Geraisy, Wasim

AU - Huebner, Eric A

AU - Woolf, Clifford J

AU - Yaron, Avraham

AU - Burlingame, Alma L

AU - Twiss, Jeffery L

AU - Fainzilber, Mike

PY - 2016/8/9

Y1 - 2016/8/9

N2 - How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin beta1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin beta1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin beta1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.

AB - How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin beta1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin beta1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin beta1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.

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DO - 10.1016/j.celrep.2016.07.005

M3 - مقالة

C2 - 27477284

SN - 2211-1247

VL - 16

SP - 1664

EP - 1676

JO - Cell Reports

JF - Cell Reports

IS - 6

ER -

Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

Abstract

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