TY - JOUR
T1 - Novel Hexb-based tools for studying microglia in the CNS
AU - Masuda, Takahiro
AU - Amann, Lukas
AU - Sankowski, Roman
AU - Staszewski, Ori
AU - Lenz, Maximilian
AU - D Errico, Paolo
AU - Snaidero, Nicolas
AU - Costa Jordão, Marta Joana
AU - Böttcher, Chotima
AU - Kierdorf, Katrin
AU - Jung, Steffen
AU - Priller, Josef
AU - Misgeld, Thomas
AU - Vlachos, Andreas
AU - Luehmann, Melanie Meyer
AU - Knobeloch, Klaus-Peter
AU - Prinz, Marco
N1 - We thank E. Barleon for excellent technical assistance and the Center for Animal Resource and Development (CARD, Kumamoto University) for providing research material. T. Masuda was supported by the KANAE Foundation for the Promotion of Medical Science and the Japan Society for the Promotion of Science (JSPS) as the JSPS Postdoctoral Fellow for Research Abroad. M.P. was supported by the Sobek Foundation, the Ernst-Jung Foundation, the German Research Foundation (DFG) (grant nos. SFB 992, SFB1160, SFB/TRR167, Reinhart-Koselleck-Grant and the Gottfried Wilhelm Leibniz-Prize) and the Ministry of Science, Research and Arts, Baden-Wuerttemberg (Sonderlinie ‘Neuroinflammation’). This study was supported by the DFG under Germany’s Excellence Strategy (grant no. CIBSS—EXC-2189, Project ID390939984). J.P. was supported by the DFG (grant nos. SFB/TRR167 B05 and B07), and the UK DRI Momentum Award. C.B. was supported by the DFG (grant no. SFB/TRR167 B05). T. Masuda, L.A., P.E., M.L., N.S., T. Misgeld, R.S., O.S., M.J.C.J., C.B., K.K., D.G., A.V. and K.P.K. conducted experiments and analyzed the data. M.P., K.P.K., S.J., M.M.L. and J.P. analyzed the data, contributed to the in vivo studies and provided mice or reagents. T. Masuda and M.P. supervised the project and wrote the manuscript, J.P. edited the manuscript.
PY - 2020/7
Y1 - 2020/7
N2 - Microglia and central nervous system (CNS)-associated macrophages (CAMs), such as perivascular and meningeal macrophages, are implicated in virtually all diseases of the CNS. However, little is known about their cell-type-specific roles in the absence of suitable tools that would allow for functional discrimination between the ontogenetically closely related microglia and CAMs. To develop a new microglia gene targeting model, we first applied massively parallel single-cell analyses to compare microglia and CAM signatures during homeostasis and disease and identified hexosaminidase subunit beta (Hexb) as a stably expressed microglia core gene, whereas other microglia core genes were substantially downregulated during pathologies. Next, we generated HexbtdTomato mice to stably monitor microglia behavior in vivo. Finally, the Hexb locus was employed for tamoxifen-inducible Cre-mediated gene manipulation in microglia and for fate mapping of microglia but not CAMs. In sum, we provide valuable new genetic tools to specifically study microglia functions in the CNS.
AB - Microglia and central nervous system (CNS)-associated macrophages (CAMs), such as perivascular and meningeal macrophages, are implicated in virtually all diseases of the CNS. However, little is known about their cell-type-specific roles in the absence of suitable tools that would allow for functional discrimination between the ontogenetically closely related microglia and CAMs. To develop a new microglia gene targeting model, we first applied massively parallel single-cell analyses to compare microglia and CAM signatures during homeostasis and disease and identified hexosaminidase subunit beta (Hexb) as a stably expressed microglia core gene, whereas other microglia core genes were substantially downregulated during pathologies. Next, we generated HexbtdTomato mice to stably monitor microglia behavior in vivo. Finally, the Hexb locus was employed for tamoxifen-inducible Cre-mediated gene manipulation in microglia and for fate mapping of microglia but not CAMs. In sum, we provide valuable new genetic tools to specifically study microglia functions in the CNS.
UR - http://www.scopus.com/inward/record.url?scp=85086761190&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41590-020-0707-4
DO - https://doi.org/10.1038/s41590-020-0707-4
M3 - مقالة
C2 - 32541832
SN - 1529-2908
VL - 21
SP - 802
EP - 815
JO - Nature Immunology
JF - Nature Immunology
IS - 7
ER -