Nonradioactive in situ hybridization on skeletal tissue sections

Yulia Shwartz, Elazar Zelzer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Spatial and temporal visualization of RNA transcripts in tissue is a key tool in studying both developmental and pathological processes. In situ hybridization is a highly sensitive method for RNA transcript detection. It is based on sequence complementation between a labeled RNA probe and the RNA transcript of interest. The labeled probe is then detected by immunohistochemical methods using an antibody conjugated to an enzyme that catalyzes the generation of chromogenic or fluorescent signals, which indicate the location of the transcript.

Original languageEnglish
Title of host publicationSkeletal Development and Repair
Subtitle of host publicationMethods and Protocols
EditorsM. Hilton
PublisherHumana Press
Pages203-215
Number of pages13
ISBN (Electronic)978-1-62703-989-5
ISBN (Print)9781627039888
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1130

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology

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