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NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera

Xavier Michalet, Arin C. Ulku, Michael A. Wayne, Shimon Weiss, Claudio Bruschini, Edoardo Charbon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

SwissSPAD3 is the latest of a family of widefield time-gated SPAD imagers developed for fluorescence lifetime imaging (FLI) applications. Its distinctive features are (i) the ability to define shorter gates than its predecessors (width W < 1 ns), (ii) support for laser repetition rates up to at least 80 MHz and (iii) a dual-gate architecture providing an effective duty cycle of 100%. We present widefield macroscopic FLI measurements of short lifetime NIR dyes, analyzed using the phasor approach. The results are compared with those previously obtained with SwissSPAD2 and to theoretical predictions.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XXIII
EditorsAmmasi Periasamy, Peter T. So, Karsten Konig
PublisherSPIE
ISBN (Electronic)9781510658738
DOIs
StatePublished - 25 Apr 2023
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences XXIII 2023 - San Francisco, United States
Duration: 29 Jan 202331 Jan 2023

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume12384

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XXIII 2023
Country/TerritoryUnited States
CitySan Francisco
Period29/01/2331/01/23

Keywords

  • NIR
  • SPAD
  • fluorescence
  • lifetime
  • phasor
  • time-gated
  • widefield

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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