@inbook{d02e5af4b91948d189c49ede1c0fdcb0,
title = "Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interacting RNA",
abstract = "Proteins with either RNA or DNA-binding motifs were shown to bind RNA. Immunoprecipitation of such proteins using antibodies and identification of the RNA-binding molecules is called RNA immunoprecipitation (RIP). The RNA precipitated with the studied protein can be detected by real-time polymerase chain reaction (PCR), microarray or sequencing. Here, we detail a method for native immunoprecipitation, without cross-linking, to isolate protein-RNA complexes followed by subsequent extraction and quantification of the co-purified RNA.",
keywords = "Antibody, Native RNA immunoprecipitation, RNA extraction, RNA-binding protein, Real-time PCR",
author = "Mai Baker and Rami Khosravi and Maayan Salton",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3191-1_8",
language = "الإنجليزيّة",
series = "Methods in Molecular Biology",
pages = "107--114",
booktitle = "Methods in Molecular Biology",
}