TY - CHAP
T1 - Nanoparticle-Assisted Stimulated Emission Depletion (STED) Super-Resolution Nanoscopy
AU - Sivan, Yonatan
AU - Sonnefraud, Yannick
N1 - Publisher Copyright: © 2017 Pan Stanford Publishing Pte. Ltd. All rights reserved.
PY - 2017
Y1 - 2017
N2 - This chapter reviews the theoretical aspects of nanoparticles (NP) - Stimulated Emission Depletion (STED)—the use of hybrid fluorescent labels consisting of a fluorescent emitter and a metal NP in STED nanoscopy. It shows that this technique has great promise in allowing for a significant improvement in all aspects of the imaging performance of STED nanoscopy, namely allowing low STED power, high resolution, or improved photostability. This variety allows one to design an NP to suit the imaging requirements and object sizes. The proposed technique relies on challenging yet demonstrated NP fabrication technology for fluorescence microscopy. In addition, it is complementary to the improvements made to STED nanoscopes; thus, it is compatible with any STED mode of operation such as continuous-wave and time-gated STED modes. A proof-of-principle experiment has confirmed the NP-STED concepts using core–shell nanostructures. The chapter examines the dependence of the improved performance on nanoshell size. Larger nanoshells provide better improvement in imaging performance.
AB - This chapter reviews the theoretical aspects of nanoparticles (NP) - Stimulated Emission Depletion (STED)—the use of hybrid fluorescent labels consisting of a fluorescent emitter and a metal NP in STED nanoscopy. It shows that this technique has great promise in allowing for a significant improvement in all aspects of the imaging performance of STED nanoscopy, namely allowing low STED power, high resolution, or improved photostability. This variety allows one to design an NP to suit the imaging requirements and object sizes. The proposed technique relies on challenging yet demonstrated NP fabrication technology for fluorescence microscopy. In addition, it is complementary to the improvements made to STED nanoscopes; thus, it is compatible with any STED mode of operation such as continuous-wave and time-gated STED modes. A proof-of-principle experiment has confirmed the NP-STED concepts using core–shell nanostructures. The chapter examines the dependence of the improved performance on nanoshell size. Larger nanoshells provide better improvement in imaging performance.
UR - http://www.scopus.com/inward/record.url?scp=85051806758&partnerID=8YFLogxK
U2 - https://doi.org/10.1201/9781315206530
DO - https://doi.org/10.1201/9781315206530
M3 - Chapter
SN - 9789814669917
SP - 247
EP - 298
BT - Plasmonics and Super-Resolution Imaging
A2 - Liu, Zhaowei
PB - Jenny Stanford Publishing
ER -