N-methylation of isopeptide bond as a strategy to resist deubiquitinases

Mahmood Haj-Yahya; Najat Eltarteer; Shimrit Ohayon; Efrat Shema; Eran Kotler; Moshe Oren; Ashraf Brik

doi:10.1002/anie.201205771

N-methylation of isopeptide bond as a strategy to resist deubiquitinases

Mahmood Haj-Yahya, Najat Eltarteer, Shimrit Ohayon, Efrat Shema, Eran Kotler, Moshe Oren, Ashraf Brik

Ben-Gurion University of the Negev, Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Stable like a rock: An efficient method to generate N-methylated isopeptide bonds has been developed. The strategy was used to generate highly stable ubiquitinated peptides and proteins that are resistant to deubiquitinases (DUBs; see scheme). Thus, the behavior of several stable ubiquitin conjugates with different DUBs was studied in-vitro and within a cellular environment.

Original language	American English
Pages (from-to)	11535-11539
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	46
DOIs	https://doi.org/10.1002/anie.201205771
State	Published - 12 Nov 2012

Keywords

N-methylation
deubiquitination
histone synthesis
stable isopeptide bond
ubiquitination

All Science Journal Classification (ASJC) codes

General Chemistry
Catalysis

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10.1002/anie.201205771

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title = "N-methylation of isopeptide bond as a strategy to resist deubiquitinases",

abstract = "Stable like a rock: An efficient method to generate N-methylated isopeptide bonds has been developed. The strategy was used to generate highly stable ubiquitinated peptides and proteins that are resistant to deubiquitinases (DUBs; see scheme). Thus, the behavior of several stable ubiquitin conjugates with different DUBs was studied in-vitro and within a cellular environment.",

keywords = "N-methylation, deubiquitination, histone synthesis, stable isopeptide bond, ubiquitination",

author = "Mahmood Haj-Yahya and Najat Eltarteer and Shimrit Ohayon and Efrat Shema and Eran Kotler and Moshe Oren and Ashraf Brik",

note = "Israel Science foundation; Israel Ministry of Science and Technology; European Research Council [293438]This work was supported by the Israel Science foundation (A. B.), the Israel Ministry of Science and Technology (M.H.-Y.), and the European Research Council grant 293438 (RUBICAN) (M.O.).",

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T1 - N-methylation of isopeptide bond as a strategy to resist deubiquitinases

AU - Haj-Yahya, Mahmood

AU - Eltarteer, Najat

AU - Ohayon, Shimrit

AU - Shema, Efrat

AU - Kotler, Eran

AU - Oren, Moshe

AU - Brik, Ashraf

N1 - Israel Science foundation; Israel Ministry of Science and Technology; European Research Council [293438]This work was supported by the Israel Science foundation (A. B.), the Israel Ministry of Science and Technology (M.H.-Y.), and the European Research Council grant 293438 (RUBICAN) (M.O.).

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Y1 - 2012/11/12

N2 - Stable like a rock: An efficient method to generate N-methylated isopeptide bonds has been developed. The strategy was used to generate highly stable ubiquitinated peptides and proteins that are resistant to deubiquitinases (DUBs; see scheme). Thus, the behavior of several stable ubiquitin conjugates with different DUBs was studied in-vitro and within a cellular environment.

AB - Stable like a rock: An efficient method to generate N-methylated isopeptide bonds has been developed. The strategy was used to generate highly stable ubiquitinated peptides and proteins that are resistant to deubiquitinases (DUBs; see scheme). Thus, the behavior of several stable ubiquitin conjugates with different DUBs was studied in-vitro and within a cellular environment.

KW - N-methylation

KW - deubiquitination

KW - histone synthesis

KW - stable isopeptide bond

KW - ubiquitination

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

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ER -

N-methylation of isopeptide bond as a strategy to resist deubiquitinases

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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