Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor

L. C. Hsiung; V. Krivitsky; V. Naddaka; Y. K. Conroy; H. Peretz-Soroka; F. Patolsky

Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor

L. C. Hsiung, V. Krivitsky, V. Naddaka, Y. K. Conroy, H. Peretz-Soroka, F. Patolsky

School of Chemistry

Tel Aviv University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We designed a redox-reactive nanowire biosensor for multiplex real-time monitoring of cellular metabolic activities. A field-effect transistor (FET) biosensor modified with 9,10-anthraquinone-2-sulfochloride detected reactive oxygen species (ROS); by ROS' oxidizing the modified monolayer, surface electron density was reduced to alter measured currents. Likewise, lactate metabolites were converted to peroxide by lactate oxidase (LOX) beforehand for sensing. Significances include: (1) the first nanowire sensing of metabolites in physiological solutions without preprocessing; (2) concentration-dependent sensing responses to H₂O₂ and lactate were verified to cover physiological concentration ranges; (3) correlating metabolic activities with proliferation rates; and (4) validating pharmaceutical mechanisms of anticancer agents.

Original language	English
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Pages	1959-1961
Number of pages	3
State	Published - 2013
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: 27 Oct 2013 → 31 Oct 2013

Publication series

Name	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume	3

Conference

Conference	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/Territory	Germany
City	Freiburg
Period	27/10/13 → 31/10/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biosensor
Field-effect transistor
Metabolites
Multiplex
Nanowire array
Real-time sensing

All Science Journal Classification (ASJC) codes

Bioengineering

Cite this

Hsiung, L. C., Krivitsky, V., Naddaka, V., Conroy, Y. K., Peretz-Soroka, H., & Patolsky, F. (2013). Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1959-1961). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3).

Hsiung, L. C. ; Krivitsky, V. ; Naddaka, V. et al. / Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 2013. pp. 1959-1961 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).

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title = "Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor",

abstract = "We designed a redox-reactive nanowire biosensor for multiplex real-time monitoring of cellular metabolic activities. A field-effect transistor (FET) biosensor modified with 9,10-anthraquinone-2-sulfochloride detected reactive oxygen species (ROS); by ROS' oxidizing the modified monolayer, surface electron density was reduced to alter measured currents. Likewise, lactate metabolites were converted to peroxide by lactate oxidase (LOX) beforehand for sensing. Significances include: (1) the first nanowire sensing of metabolites in physiological solutions without preprocessing; (2) concentration-dependent sensing responses to H2O2 and lactate were verified to cover physiological concentration ranges; (3) correlating metabolic activities with proliferation rates; and (4) validating pharmaceutical mechanisms of anticancer agents.",

keywords = "Biosensor, Field-effect transistor, Metabolites, Multiplex, Nanowire array, Real-time sensing",

author = "Hsiung, \{L. C.\} and V. Krivitsky and V. Naddaka and Conroy, \{Y. K.\} and H. Peretz-Soroka and F. Patolsky",

year = "2013",

language = "الإنجليزيّة",

isbn = "9781632666246",

series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",

pages = "1959--1961",

booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",

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Hsiung, LC, Krivitsky, V, Naddaka, V, Conroy, YK, Peretz-Soroka, H & Patolsky, F 2013, Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 3, pp. 1959-1961, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 27/10/13.

Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor. / Hsiung, L. C.; Krivitsky, V.; Naddaka, V. et al.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 2013. p. 1959-1961 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor

AU - Hsiung, L. C.

AU - Krivitsky, V.

AU - Naddaka, V.

AU - Conroy, Y. K.

AU - Peretz-Soroka, H.

AU - Patolsky, F.

PY - 2013

Y1 - 2013

N2 - We designed a redox-reactive nanowire biosensor for multiplex real-time monitoring of cellular metabolic activities. A field-effect transistor (FET) biosensor modified with 9,10-anthraquinone-2-sulfochloride detected reactive oxygen species (ROS); by ROS' oxidizing the modified monolayer, surface electron density was reduced to alter measured currents. Likewise, lactate metabolites were converted to peroxide by lactate oxidase (LOX) beforehand for sensing. Significances include: (1) the first nanowire sensing of metabolites in physiological solutions without preprocessing; (2) concentration-dependent sensing responses to H2O2 and lactate were verified to cover physiological concentration ranges; (3) correlating metabolic activities with proliferation rates; and (4) validating pharmaceutical mechanisms of anticancer agents.

AB - We designed a redox-reactive nanowire biosensor for multiplex real-time monitoring of cellular metabolic activities. A field-effect transistor (FET) biosensor modified with 9,10-anthraquinone-2-sulfochloride detected reactive oxygen species (ROS); by ROS' oxidizing the modified monolayer, surface electron density was reduced to alter measured currents. Likewise, lactate metabolites were converted to peroxide by lactate oxidase (LOX) beforehand for sensing. Significances include: (1) the first nanowire sensing of metabolites in physiological solutions without preprocessing; (2) concentration-dependent sensing responses to H2O2 and lactate were verified to cover physiological concentration ranges; (3) correlating metabolic activities with proliferation rates; and (4) validating pharmaceutical mechanisms of anticancer agents.

KW - Biosensor

KW - Field-effect transistor

KW - Metabolites

KW - Multiplex

KW - Nanowire array

KW - Real-time sensing

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M3 - منشور من مؤتمر

SN - 9781632666246

T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

SP - 1959

EP - 1961

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Y2 - 27 October 2013 through 31 October 2013

ER -

Hsiung LC, Krivitsky V, Naddaka V, Conroy YK, Peretz-Soroka H, Patolsky F. Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 2013. p. 1959-1961. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).

Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor

Abstract

Publication series

Conference

UN SDGs

Keywords

All Science Journal Classification (ASJC) codes

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