@inproceedings{afb3a668bda9465c9ced784a752507fd,
title = "Multiplex real-time monitoring of cellular metabolic activity using a redox-reactive nanowire biosensor",
abstract = "We designed a redox-reactive nanowire biosensor for multiplex real-time monitoring of cellular metabolic activities. A field-effect transistor (FET) biosensor modified with 9,10-anthraquinone-2-sulfochloride detected reactive oxygen species (ROS); by ROS' oxidizing the modified monolayer, surface electron density was reduced to alter measured currents. Likewise, lactate metabolites were converted to peroxide by lactate oxidase (LOX) beforehand for sensing. Significances include: (1) the first nanowire sensing of metabolites in physiological solutions without preprocessing; (2) concentration-dependent sensing responses to H2O2 and lactate were verified to cover physiological concentration ranges; (3) correlating metabolic activities with proliferation rates; and (4) validating pharmaceutical mechanisms of anticancer agents.",
keywords = "Biosensor, Field-effect transistor, Metabolites, Multiplex, Nanowire array, Real-time sensing",
author = "Hsiung, {L. C.} and V. Krivitsky and V. Naddaka and Conroy, {Y. K.} and H. Peretz-Soroka and F. Patolsky",
year = "2013",
language = "الإنجليزيّة",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
pages = "1959--1961",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}
