Multifunctional Nanoscale Platform for the Study of T Cell Receptor Segregation

Esti Toledo, Muhammed Iraqi, Ashish Pandey, Sivan Tzadka, Guillaume Le Saux, Angel Porgador, Mark Schvartzman

Research output: Contribution to journalArticlepeer-review


T cells respond not only to biochemical stimuli transmitted through their activating, costimulatory, and inhibitory receptors but also to biophysical aspects of their environment, including the receptors’ spatial arrangement. While these receptors form nanoclusters that can either colocalize or segregate, the roles of these colocalization and segregation remain unclear. Deciphering these roles requires a nanoscale platform with independent and simultaneous spatial control of multiple types of receptors. Herein, using a straightforward and modular fabrication process, we engineered a tunable nanoscale chip used as a platform for T cell stimulation, allowing spatial control over the clustering and segregation of activating, costimulatory, and inhibitory receptors. Using this platform, we showed that, upon blocked inhibition, cells became sensitive to changes in the nanoscale ligand configuration. The nanofabrication methodology described here opens a pathway to numerous studies, which will produce an important insight into the molecular mechanism of T cell activation. This insight is essential for the fundamental understanding of our immune system as well as for the rational design of future immunotherapies.

Original languageAmerican English
Pages (from-to)28968-28975
Number of pages8
JournalACS Omega
Issue number32
StatePublished - 15 Aug 2023

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Chemical Engineering


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