Multi-harmonic structured illumination-based optical diffraction tomography

Ruihua Liu; Kai Wen; Jiaoyue Li; Ying Ma; Juanjuan Zheng; Sha An; Junwei Min; Zeev Zalevsky; Baoli Yao; Peng Gao

doi:10.1364/ao.508138

Multi-harmonic structured illumination-based optical diffraction tomography

Ruihua Liu, Kai Wen, Jiaoyue Li, Ying Ma, Juanjuan Zheng, Sha An, Junwei Min, Zeev Zalevsky, Baoli Yao, Peng Gao

Bar-Ilan University - The Alexander Kofkin Faculty of Engineering

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illuminationbased optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7% in 3D RI imaging.Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 mpolystyrene (PS) microspheres and biological samples.

Original language	English
Pages (from-to)	9199-9206
Number of pages	8
Journal	APPLIED OPTICS
Volume	62
Issue number	35
DOIs	https://doi.org/10.1364/ao.508138
State	Published - 10 Dec 2023

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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title = "Multi-harmonic structured illumination-based optical diffraction tomography",

abstract = "Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illuminationbased optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7% in 3D RI imaging.Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 mpolystyrene (PS) microspheres and biological samples.",

author = "Ruihua Liu and Kai Wen and Jiaoyue Li and Ying Ma and Juanjuan Zheng and Sha An and Junwei Min and Zeev Zalevsky and Baoli Yao and Peng Gao",

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day = "10",

doi = "10.1364/ao.508138",

language = "الإنجليزيّة",

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T1 - Multi-harmonic structured illumination-based optical diffraction tomography

AU - Liu, Ruihua

AU - Wen, Kai

AU - Li, Jiaoyue

AU - Ma, Ying

AU - Zheng, Juanjuan

AU - An, Sha

AU - Min, Junwei

AU - Zalevsky, Zeev

AU - Yao, Baoli

AU - Gao, Peng

PY - 2023/12/10

Y1 - 2023/12/10

N2 - Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illuminationbased optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7% in 3D RI imaging.Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 mpolystyrene (PS) microspheres and biological samples.

AB - Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illuminationbased optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7% in 3D RI imaging.Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 mpolystyrene (PS) microspheres and biological samples.

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DO - 10.1364/ao.508138

M3 - مقالة

C2 - 38108690

SN - 1559-128X

VL - 62

SP - 9199

EP - 9206

JO - APPLIED OPTICS

JF - APPLIED OPTICS

IS - 35

ER -

Multi-harmonic structured illumination-based optical diffraction tomography

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All Science Journal Classification (ASJC) codes

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