TY - UNPB
T1 - Morphometric reconstructions atlas shows insult-driven plasticity in cortical VIP/ChAT interneurons
AU - Yayon, Nadav
AU - Amsalem, Oren
AU - Dudai, Amir
AU - Yakov, Or
AU - Adam, Gil
AU - Tessier-Lavigne, Marc
AU - Renier, Nicolas
AU - Segev, Idan
AU - London, Michael
AU - Soreq, Hermona
PY - 2020
Y1 - 2020
N2 - We developed an automatic morphometric reconstruction pipeline, Pop-Rec, and used it to study the morphologies of cortical cholinergic VIP/ChAT interneurons (VChIs). Cholinergic networks control high cognitive functions, but their local modulation and stress-driven plasticity patterns remained elusive. Reconstructing thousands of local VChIs registered to their exact coordinates in multiple cleared murine cortices highlighted distinct populations of bipolar and multipolar VChIs which differed in their dendritic spatial organization. Following mild unilateral whisker deprivation, Pop-Rec found both ipsi-and contra-lateral VChI dendriticarborization changes. Furthermore, RNA-seq of FACS-sorted VChIs showed differentiallyexpressed dendritic, synapse and axon-modulating transcripts in whisker-deprived mice.Indicating novel steady-state morphological roles, those genes also clustered distinctly innaïve single cell VChIs. This VChIs “morpheome” atlas is the first example of unbiased analysisof neuronal populations and holds the possibility to compare neuronal structure-function relationships across experimental conditions.
AB - We developed an automatic morphometric reconstruction pipeline, Pop-Rec, and used it to study the morphologies of cortical cholinergic VIP/ChAT interneurons (VChIs). Cholinergic networks control high cognitive functions, but their local modulation and stress-driven plasticity patterns remained elusive. Reconstructing thousands of local VChIs registered to their exact coordinates in multiple cleared murine cortices highlighted distinct populations of bipolar and multipolar VChIs which differed in their dendritic spatial organization. Following mild unilateral whisker deprivation, Pop-Rec found both ipsi-and contra-lateral VChI dendriticarborization changes. Furthermore, RNA-seq of FACS-sorted VChIs showed differentiallyexpressed dendritic, synapse and axon-modulating transcripts in whisker-deprived mice.Indicating novel steady-state morphological roles, those genes also clustered distinctly innaïve single cell VChIs. This VChIs “morpheome” atlas is the first example of unbiased analysisof neuronal populations and holds the possibility to compare neuronal structure-function relationships across experimental conditions.
U2 - https://doi.org/10.1101/2020.08.25.263178
DO - https://doi.org/10.1101/2020.08.25.263178
M3 - Preprint
VL - 263178
SP - 1
EP - 29
BT - Morphometric reconstructions atlas shows insult-driven plasticity in cortical VIP/ChAT interneurons
ER -