Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

Yaarit Adamovich; Saar Ezagouri; Gad Asher

doi:https://doi.org/10.1016/j.xpro.2021.100331

Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

Yaarit Adamovich, Saar Ezagouri, Gad Asher

Department of Biomolecular Sciences

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

There is growing interest in medicine and sports in uncovering exercise modifiers that enhance or limit exercise capacity. Here, we detail a protocol for testing the daytime effect on running capacity in mice using a moderate intensity treadmill effort test. Instructions for dissecting soleus, gastrocnemius plantaris, and quadriceps muscles for further analysis are provided as well. This experimental setup is optimized for addressing questions regarding the involvement of daytime and circadian clocks in regulating exercise capacity. For complete details on the use and execution of this protocol, please refer to Ezagouri et al. (2019)

Original language	English
Article number	100331
Number of pages	14
Journal	STAR Protocols
Volume	2
Issue number	1
Early online date	5 Feb 2021
DOIs	https://doi.org/10.1016/j.xpro.2021.100331
State	Published - 19 Mar 2021

All Science Journal Classification (ASJC) codes

General Immunology and Microbiology
General Biochemistry,Genetics and Molecular Biology
General Neuroscience
General Medicine

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https://doi.org/10.1016/j.xpro.2021.100331License: CC BY

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Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

Abstract

All Science Journal Classification (ASJC) codes

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