Modified ECM-Based Bioink for 3D Printing of Multi-Scale Vascular Networks

Roni Cohen, Ester Sapir Baruch, Itai Cabilly, Assaf Shapira, Tal Dvir

Research output: Contribution to journalArticlepeer-review

Abstract

The survival and function of tissues depend on appropriate vascularization. Blood vessels of the tissues supply oxygen, and nutrients and remove waste and byproducts. Incorporating blood vessels into engineered tissues is essential for overcoming diffusion limitations, improving tissue function, and thus facilitating the fabrication of thick tissues. Here, we present a modified ECM bioink, with enhanced mechanical properties and endothelial cell-specific adhesion motifs, to serve as a building material for 3D printing of a multiscale blood vessel network. The bioink is composed of natural ECM and alginate conjugated with a laminin adhesion molecule motif (YIGSR). The hybrid hydrogel was characterized for its mechanical properties, biochemical content, and ability to interact with endothelial cells. The pristine and modified hydrogels were mixed with induced pluripotent stem cells derived endothelial cells (iPSCs-ECs) and used to print large blood vessels with capillary beds in between.

Original languageEnglish
Article number792
JournalGels
Volume9
Issue number10
DOIs
StatePublished - Oct 2023

Keywords

  • 3D printing
  • blood vessels
  • iPSCs
  • tissue engineering
  • vascularization

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Polymers and Plastics

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