Mixed alkanethiol monolayers on submicrometric gold patterns: A controlled platform for studying cell-ligand interactions

Rami Fishler, Arbel Artzy-Schnirman, Elad Peer, Ron Wolchinsky, Reuven Brener, Tova Waks, Zelig Eshhar, Yoram Reiter, Uri Sivan

Research output: Contribution to journalArticlepeer-review

Abstract

Nanoscale organization of surface ligands often has a critical effect on cell-surface interactions. We have developed an experimental system that allows a high degree of control over the 2-D spatial distribution of ligands. As a proof of concept, we used the developed system to study how T-cell activation is independently affected by antigen density and antigen amount per cell. Arrays of submicrometer gold islands at varying surface coverage were defined on silicon by electron beam lithography (EBL). The gold islands were functionalized with alkanethiol self-assembled monolayers (SAMs) containing a small antigen, 2,4,6-trinotrophenyl (TNP), at various densities. Genetically engineered T-cell hybridomas expressing TNP-specific chimeric T-cell antigen receptor (CAR) were cultured on the SAMs, and their activation was assessed by IL-2 secretion and CD69 expression. It was found that, at constant antigen density, activation increased monotonically with the amount of antigen, while at constant antigen amount activation was maximal at an intermediate antigen density, whose value was independent of the amount of antigen.

Original languageEnglish
Pages (from-to)4992-4996
Number of pages5
JournalNano Letters
Volume12
Issue number9
DOIs
StatePublished - 12 Sep 2012

Keywords

  • T-cell activation
  • X-ray photoelectron spectroscopy of SAMs
  • cell interaction with SAM
  • immobilized antigen
  • patterned antigen surface

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Condensed Matter Physics
  • Mechanical Engineering
  • Bioengineering
  • General Materials Science

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