Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

Inna Chmelnitsky; Maya Shklyar; Azita Leavitt; Evgeniya Sadovsky; Shiri Navon-Venezia; Maayan Ben Dalak; Rotem Edgar; Yehuda Carmeli

doi:10.1016/j.diagmicrobio.2014.03.008

Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

Inna Chmelnitsky
, Maya Shklyar
, Azita Leavitt
, Evgeniya Sadovsky
, Shiri Navon-Venezia
, Maayan Ben Dalak
, Rotem Edgar
, Yehuda Carmeli

Ariel University

Research output: Contribution to journal › Article › peer-review

Abstract

We performed comparative sequence analysis of 3 bla_KPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The bla_KPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional bla_KPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the bla_KPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.

Original language	English
Pages (from-to)	255-260
Number of pages	6
Journal	Diagnostic Microbiology and Infectious Disease
Volume	79
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2014.03.008
State	Published - Jun 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Enterobacteriaceae
KPC
P2 promoter
Tn4401

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2014.03.008

Cite this

@article{c16be510d232428c9feb6d85ca71d3b7,

title = "Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics",

abstract = "We performed comparative sequence analysis of 3 blaKPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The blaKPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional blaKPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the blaKPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.",

keywords = "Enterobacteriaceae, KPC, P2 promoter, Tn4401",

author = "Inna Chmelnitsky and Maya Shklyar and Azita Leavitt and Evgeniya Sadovsky and Shiri Navon-Venezia and \{Ben Dalak\}, Maayan and Rotem Edgar and Yehuda Carmeli",

year = "2014",

month = jun,

doi = "10.1016/j.diagmicrobio.2014.03.008",

language = "الإنجليزيّة",

volume = "79",

pages = "255--260",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

AU - Chmelnitsky, Inna

AU - Shklyar, Maya

AU - Leavitt, Azita

AU - Sadovsky, Evgeniya

AU - Navon-Venezia, Shiri

AU - Ben Dalak, Maayan

AU - Edgar, Rotem

AU - Carmeli, Yehuda

PY - 2014/6

Y1 - 2014/6

N2 - We performed comparative sequence analysis of 3 blaKPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The blaKPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional blaKPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the blaKPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.

AB - We performed comparative sequence analysis of 3 blaKPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The blaKPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional blaKPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the blaKPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.

KW - Enterobacteriaceae

KW - KPC

KW - P2 promoter

KW - Tn4401

UR - https://www.scopus.com/pages/publications/84900497226

U2 - 10.1016/j.diagmicrobio.2014.03.008

DO - 10.1016/j.diagmicrobio.2014.03.008

M3 - مقالة

C2 - 24743043

SN - 0732-8893

VL - 79

SP - 255

EP - 260

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

ER -

Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this