Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

Inna Chmelnitsky; Maya Shklyar; Azita Leavitt; Evgeniya Sadovsky; Shiri Navon-Venezia; Maayan Ben Dalak; Rotem Edgar; Yehuda Carmeli

doi:https://doi.org/10.1016/j.diagmicrobio.2014.03.008

Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

Inna Chmelnitsky, Maya Shklyar, Azita Leavitt, Evgeniya Sadovsky, Shiri Navon-Venezia, Maayan Ben Dalak, Rotem Edgar, Yehuda Carmeli

Ariel University

Research output: Contribution to journal › Article › peer-review

Abstract

We performed comparative sequence analysis of 3 bla_KPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The bla_KPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional bla_KPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the bla_KPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.

Original language	English
Pages (from-to)	255-260
Number of pages	6
Journal	Diagnostic Microbiology and Infectious Disease
Volume	79
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2014.03.008
State	Published - Jun 2014

Keywords

Enterobacteriaceae
KPC
P2 promoter
Tn4401

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.1016/j.diagmicrobio.2014.03.008

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title = "Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics",

abstract = "We performed comparative sequence analysis of 3 blaKPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The blaKPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional blaKPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the blaKPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.",

keywords = "Enterobacteriaceae, KPC, P2 promoter, Tn4401",

author = "Inna Chmelnitsky and Maya Shklyar and Azita Leavitt and Evgeniya Sadovsky and Shiri Navon-Venezia and {Ben Dalak}, Maayan and Rotem Edgar and Yehuda Carmeli",

year = "2014",

month = jun,

doi = "https://doi.org/10.1016/j.diagmicrobio.2014.03.008",

language = "الإنجليزيّة",

volume = "79",

pages = "255--260",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

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TY - JOUR

T1 - Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

AU - Chmelnitsky, Inna

AU - Shklyar, Maya

AU - Leavitt, Azita

AU - Sadovsky, Evgeniya

AU - Navon-Venezia, Shiri

AU - Ben Dalak, Maayan

AU - Edgar, Rotem

AU - Carmeli, Yehuda

PY - 2014/6

Y1 - 2014/6

N2 - We performed comparative sequence analysis of 3 blaKPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The blaKPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional blaKPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the blaKPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.

AB - We performed comparative sequence analysis of 3 blaKPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The blaKPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional blaKPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the blaKPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.

KW - Enterobacteriaceae

KW - KPC

KW - P2 promoter

KW - Tn4401

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U2 - https://doi.org/10.1016/j.diagmicrobio.2014.03.008

DO - https://doi.org/10.1016/j.diagmicrobio.2014.03.008

M3 - مقالة

C2 - 24743043

SN - 0732-8893

VL - 79

SP - 255

EP - 260

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

ER -

Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

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