Misincorporation signatures for detecting modifications in mRNA: Not as simple as it sounds

Research output: Contribution to journalReview articlepeer-review

Abstract

Post-transcriptional modification on mRNA has become a field of intense interest in recent years, and next-generation sequencing based technologies are constantly emerging to detect an increasing number of modifications at a transcriptome-wide level. Some of these approaches are based on identification of misincorporation events induced by reverse transcriptase at modified sites. Although conceptually trivial, sensitive and specific identification of such events is a challenge prone to a surprising number of artifacts, which can lead to substantially inflated estimates of the abundance of diverse modifications. Here we discuss the sources of some of these artifacts and delineate approaches to overcome them.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalMethods
Volume156
DOIs
StatePublished - 1 Mar 2019

Keywords

  • Epitranscriptome
  • Misincorporation signatures
  • Next generation sequencing
  • RNA modification

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology
  • Molecular Biology

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