MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

Lihi Ninio-Many; Hadas Grossman; Mattan Levi; Sofia Zilber; Ilan Tsarfaty; Noam Shomron; Anna Tuvar; Dana Chuderland; Salomon M. Stemmer; Irit Ben-Aharon; Ruth Shalgi

doi:10.18632/oncoscience.29

MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

Lihi Ninio-Many, Hadas Grossman, Mattan Levi, Sofia Zilber, Ilan Tsarfaty, Noam Shomron, Anna Tuvar, Dana Chuderland, Salomon M. Stemmer, Irit Ben-Aharon, Ruth Shalgi

Tel Aviv University, Technion - Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.

Original language	English
Pages (from-to)	250-261
Number of pages	12
Journal	Oncoscience
Volume	1
Issue number	4
DOIs	https://doi.org/10.18632/oncoscience.29
State	Published - 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Actin cytoskeleton
EMT
Fyn
Live imaging
MiR-125a-3p
Migration
Prostate cancer

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

Access to Document

10.18632/oncoscience.29

Cite this

@article{3c8024f40809471298d7212e1754e412,

title = "MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells",

abstract = "Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.",

keywords = "Actin cytoskeleton, EMT, Fyn, Live imaging, MiR-125a-3p, Migration, Prostate cancer",

author = "Lihi Ninio-Many and Hadas Grossman and Mattan Levi and Sofia Zilber and Ilan Tsarfaty and Noam Shomron and Anna Tuvar and Dana Chuderland and Stemmer, \{Salomon M.\} and Irit Ben-Aharon and Ruth Shalgi",

note = "Funding Information: This work was supported by a grant from the Israel Science Foundation [grant number 261/09 to R.S.].",

year = "2014",

doi = "10.18632/oncoscience.29",

language = "الإنجليزيّة",

volume = "1",

pages = "250--261",

journal = "Oncoscience",

issn = "2331-4737",

publisher = "Impact Journals LLC",

number = "4",

}

TY - JOUR

T1 - MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

AU - Ninio-Many, Lihi

AU - Grossman, Hadas

AU - Levi, Mattan

AU - Zilber, Sofia

AU - Tsarfaty, Ilan

AU - Shomron, Noam

AU - Tuvar, Anna

AU - Chuderland, Dana

AU - Stemmer, Salomon M.

AU - Ben-Aharon, Irit

AU - Shalgi, Ruth

N1 - Funding Information: This work was supported by a grant from the Israel Science Foundation [grant number 261/09 to R.S.].

PY - 2014

Y1 - 2014

N2 - Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.

AB - Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.

KW - Actin cytoskeleton

KW - EMT

KW - Fyn

KW - Live imaging

KW - MiR-125a-3p

KW - Migration

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=84921405487&partnerID=8YFLogxK

U2 - 10.18632/oncoscience.29

DO - 10.18632/oncoscience.29

M3 - مقالة

SN - 2331-4737

VL - 1

SP - 250

EP - 261

JO - Oncoscience

JF - Oncoscience

IS - 4

ER -

MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

Abstract

UN SDGs

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this