Abstract
Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.
Original language | English |
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Pages (from-to) | 250-261 |
Number of pages | 12 |
Journal | Oncoscience |
Volume | 1 |
Issue number | 4 |
DOIs | |
State | Published - 2014 |
Keywords
- Actin cytoskeleton
- EMT
- Fyn
- Live imaging
- MiR-125a-3p
- Migration
- Prostate cancer
All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research