TY - JOUR
T1 - MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis
AU - Murugaiyan, Gopal
AU - da Cunha, Andre Pires
AU - Ajay, Amrendra K.
AU - Joller, Nicole
AU - Garo, Lucien P.
AU - Kumaradevan, Sowmiya
AU - Yosef, Nir
AU - Vaidya, Vishal S.
AU - Weiner, Howard L.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Accumulation of IL-17 producing Th17 cells is associated with the development of multiple autoimmune diseases; however, the contribution of microRNA (miRNA) pathways to the intrinsic control of Th17 development remains unclear. Here, we demonstrated that miR-21 expression is elevated in Th17 cells and that mice lacking miR-21 have a defect in Th17 differentiation and are resistant to experimental autoimmune encephalomyelitis (EAE). Furthermore, we determined that miR-21 promotes Th17 differentiation by targeting and depleting SMAD-7, a negative regulator of TGF-beta signaling. Moreover, the decreases in Th17 differentiation in miR-21 deficient T cells were associated with defects in SMAD-2/3 activation and IL-2 suppression. Finally, we found that treatment of WT mice with an anti miR-21 oligonucleotide reduced the clinical severity of EAE, which was associated with a decrease in Th17 cells. Thus, we have characterized a T cell intrinsic miRNA pathway that enhances TGF-beta signaling, limits the autocrine inhibitory effects of IL-2, and thereby promotes Th17 differentiation and autoimmunity.
AB - Accumulation of IL-17 producing Th17 cells is associated with the development of multiple autoimmune diseases; however, the contribution of microRNA (miRNA) pathways to the intrinsic control of Th17 development remains unclear. Here, we demonstrated that miR-21 expression is elevated in Th17 cells and that mice lacking miR-21 have a defect in Th17 differentiation and are resistant to experimental autoimmune encephalomyelitis (EAE). Furthermore, we determined that miR-21 promotes Th17 differentiation by targeting and depleting SMAD-7, a negative regulator of TGF-beta signaling. Moreover, the decreases in Th17 differentiation in miR-21 deficient T cells were associated with defects in SMAD-2/3 activation and IL-2 suppression. Finally, we found that treatment of WT mice with an anti miR-21 oligonucleotide reduced the clinical severity of EAE, which was associated with a decrease in Th17 cells. Thus, we have characterized a T cell intrinsic miRNA pathway that enhances TGF-beta signaling, limits the autocrine inhibitory effects of IL-2, and thereby promotes Th17 differentiation and autoimmunity.
U2 - https://doi.org/10.1172/JCI74347
DO - https://doi.org/10.1172/JCI74347
M3 - مقالة
C2 - 25642768
SN - 0021-9738
VL - 125
SP - 1069
EP - 1080
JO - The Journal of Clinical Investigation
JF - The Journal of Clinical Investigation
IS - 3
ER -