Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy

YongKeun Park, Catherine Best, Thorsten Auth, Nir S. Gov, Samuel Safran, Gabriel Popescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have quantitatively and systemically measured the morphologies and dynamics of fluctuations in human RBC membranes using a full-field laser interferometry technique that accurately measures dynamic membrane fluctuations. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates nonequilibrium dynamic fluctuations in the RBC membrane and that these fluctuations are highly correlated with specific regions in the biconcave shape of RBCs. Spatial analysis reveals that these nonequilibrium membrane fluctuations are enhanced at the scale of the spectrin mesh size. Our results indicate the presence of dynamic remodeling in the RBC membrane cortex powered by ATP, which results in nonequilibrium membrane fluctuations. (This conference proceeding paper is primary based on our recent publication - please refer to YK Park et al., Proc. Nat. Acad. Sci., 107, 1289 (2010) for details.)

Original languageEnglish
Title of host publicationIMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX
EditorsDL Farkas, DV Nicolau, RC Leif
PublisherSPIE
Number of pages9
Volume7902
ISBN (Print)9780819484390
DOIs
StatePublished - 22 Feb 2011
EventConference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX - San Francisco, United States
Duration: 22 Jan 201125 Jan 2011

Publication series

NameProceedings of SPIE
Volume7902
ISSN (Print)0277-786X

Conference

ConferenceConference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Country/TerritoryUnited States
CitySan Francisco
Period22/01/1125/01/11

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials

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