Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy

YongKeun Park; Catherine Best; Thorsten Auth; Nir S. Gov; Samuel Safran; Gabriel Popescu

doi:10.1117/12.874768

Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy

YongKeun Park, Catherine Best, Thorsten Auth, Nir S. Gov, Samuel Safran, Gabriel Popescu

Department of Chemical and Biological Physics

Weizmann Institute of Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have quantitatively and systemically measured the morphologies and dynamics of fluctuations in human RBC membranes using a full-field laser interferometry technique that accurately measures dynamic membrane fluctuations. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates nonequilibrium dynamic fluctuations in the RBC membrane and that these fluctuations are highly correlated with specific regions in the biconcave shape of RBCs. Spatial analysis reveals that these nonequilibrium membrane fluctuations are enhanced at the scale of the spectrin mesh size. Our results indicate the presence of dynamic remodeling in the RBC membrane cortex powered by ATP, which results in nonequilibrium membrane fluctuations. (This conference proceeding paper is primary based on our recent publication - please refer to YK Park et al., Proc. Nat. Acad. Sci., 107, 1289 (2010) for details.)

Original language	English
Title of host publication	IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX
Editors	DL Farkas, DV Nicolau, RC Leif
Publisher	SPIE
Number of pages	9
Volume	7902
ISBN (Print)	9780819484390
DOIs	https://doi.org/10.1117/12.874768
State	Published - 22 Feb 2011
Event	Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX - San Francisco, United States Duration: 22 Jan 2011 → 25 Jan 2011

Publication series

Name	Proceedings of SPIE
Volume	7902
ISSN (Print)	0277-786X

Conference

Conference	Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Country/Territory	United States
City	San Francisco
Period	22/01/11 → 25/01/11

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging
Biomaterials

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10.1117/12.874768

Cite this

Park, Y., Best, C., Auth, T., Gov, N. S., Safran, S., & Popescu, G. (2011). Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy. In DL. Farkas, DV. Nicolau, & RC. Leif (Eds.), IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX (Vol. 7902). Article 79021M (Proceedings of SPIE; Vol. 7902). SPIE. https://doi.org/10.1117/12.874768

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title = "Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy",

abstract = "We have quantitatively and systemically measured the morphologies and dynamics of fluctuations in human RBC membranes using a full-field laser interferometry technique that accurately measures dynamic membrane fluctuations. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates nonequilibrium dynamic fluctuations in the RBC membrane and that these fluctuations are highly correlated with specific regions in the biconcave shape of RBCs. Spatial analysis reveals that these nonequilibrium membrane fluctuations are enhanced at the scale of the spectrin mesh size. Our results indicate the presence of dynamic remodeling in the RBC membrane cortex powered by ATP, which results in nonequilibrium membrane fluctuations. (This conference proceeding paper is primary based on our recent publication - please refer to YK Park et al., Proc. Nat. Acad. Sci., 107, 1289 (2010) for details.)",

author = "YongKeun Park and Catherine Best and Thorsten Auth and Gov, {Nir S.} and Samuel Safran and Gabriel Popescu",

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Park, Y, Best, C, Auth, T, Gov, NS , Safran, S & Popescu, G 2011, Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy. in DL Farkas, DV Nicolau & RC Leif (eds), IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX. vol. 7902, 79021M, Proceedings of SPIE, vol. 7902, SPIE, Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, San Francisco, California, United States, 22/01/11. https://doi.org/10.1117/12.874768

Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy. / Park, YongKeun; Best, Catherine; Auth, Thorsten et al.
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX. ed. / DL Farkas; DV Nicolau; RC Leif. Vol. 7902 SPIE, 2011. 79021M (Proceedings of SPIE; Vol. 7902).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy

AU - Park, YongKeun

AU - Best, Catherine

AU - Auth, Thorsten

AU - Gov, Nir S.

AU - Safran, Samuel

AU - Popescu, Gabriel

N1 - NA

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N2 - We have quantitatively and systemically measured the morphologies and dynamics of fluctuations in human RBC membranes using a full-field laser interferometry technique that accurately measures dynamic membrane fluctuations. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates nonequilibrium dynamic fluctuations in the RBC membrane and that these fluctuations are highly correlated with specific regions in the biconcave shape of RBCs. Spatial analysis reveals that these nonequilibrium membrane fluctuations are enhanced at the scale of the spectrin mesh size. Our results indicate the presence of dynamic remodeling in the RBC membrane cortex powered by ATP, which results in nonequilibrium membrane fluctuations. (This conference proceeding paper is primary based on our recent publication - please refer to YK Park et al., Proc. Nat. Acad. Sci., 107, 1289 (2010) for details.)

AB - We have quantitatively and systemically measured the morphologies and dynamics of fluctuations in human RBC membranes using a full-field laser interferometry technique that accurately measures dynamic membrane fluctuations. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates nonequilibrium dynamic fluctuations in the RBC membrane and that these fluctuations are highly correlated with specific regions in the biconcave shape of RBCs. Spatial analysis reveals that these nonequilibrium membrane fluctuations are enhanced at the scale of the spectrin mesh size. Our results indicate the presence of dynamic remodeling in the RBC membrane cortex powered by ATP, which results in nonequilibrium membrane fluctuations. (This conference proceeding paper is primary based on our recent publication - please refer to YK Park et al., Proc. Nat. Acad. Sci., 107, 1289 (2010) for details.)

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ER -

Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy

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