Measuring transcription dynamics in living cells using a photobleaching approach

Hodaya Hochberg; Yehuda Brody; Yaron Shav-Tal

doi:10.1016/j.ymeth.2017.04.007

Measuring transcription dynamics in living cells using a photobleaching approach

Hodaya Hochberg, Yehuda Brody, Yaron Shav-Tal

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Contribution to journal › Review article › peer-review

Abstract

The transcriptional kinetics of RNA polymerase II, the enzyme responsible for mRNA transcription in the nucleoplasm, can be modulated by a variety of factors. It is therefore important to establish experimental systems that will enable the readout of transcription kinetics of specific genes as they occur in real time within individual cells. This can be performed by implementing fluorescent tagging of the mRNA under live-cell conditions. This chapter describes how to generate fluorescently tagged genes and mRNA, and how a photobleaching approach can produce information on mRNA transcription kinetics.

Original language	English
Pages (from-to)	58-64
Number of pages	7
Journal	Methods
Volume	120
DOIs	https://doi.org/10.1016/j.ymeth.2017.04.007
State	Published - 1 May 2017

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology
Molecular Biology

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10.1016/j.ymeth.2017.04.007

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abstract = "The transcriptional kinetics of RNA polymerase II, the enzyme responsible for mRNA transcription in the nucleoplasm, can be modulated by a variety of factors. It is therefore important to establish experimental systems that will enable the readout of transcription kinetics of specific genes as they occur in real time within individual cells. This can be performed by implementing fluorescent tagging of the mRNA under live-cell conditions. This chapter describes how to generate fluorescently tagged genes and mRNA, and how a photobleaching approach can produce information on mRNA transcription kinetics.",

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N2 - The transcriptional kinetics of RNA polymerase II, the enzyme responsible for mRNA transcription in the nucleoplasm, can be modulated by a variety of factors. It is therefore important to establish experimental systems that will enable the readout of transcription kinetics of specific genes as they occur in real time within individual cells. This can be performed by implementing fluorescent tagging of the mRNA under live-cell conditions. This chapter describes how to generate fluorescently tagged genes and mRNA, and how a photobleaching approach can produce information on mRNA transcription kinetics.

AB - The transcriptional kinetics of RNA polymerase II, the enzyme responsible for mRNA transcription in the nucleoplasm, can be modulated by a variety of factors. It is therefore important to establish experimental systems that will enable the readout of transcription kinetics of specific genes as they occur in real time within individual cells. This can be performed by implementing fluorescent tagging of the mRNA under live-cell conditions. This chapter describes how to generate fluorescently tagged genes and mRNA, and how a photobleaching approach can produce information on mRNA transcription kinetics.

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DO - 10.1016/j.ymeth.2017.04.007

M3 - مقالة مرجعية

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VL - 120

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JO - Methods

JF - Methods

ER -

Measuring transcription dynamics in living cells using a photobleaching approach

Abstract

All Science Journal Classification (ASJC) codes

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