@inbook{2307ac965ad2407f8aba48184a118e02,
title = "Measuring cyclic diguanylate (c-di-GMP)-specific phosphodiesterase activity using the MANT-c-di-GMP assay",
abstract = "The second messenger, cyclic diguanylate (c-di-GMP), regulates a variety of bacterial cellular and social behaviors. A key determinant of c-di-GMP levels in cells is its degradation by c-di-GMP-specific phosphodiesterases (PDEs). Here, we describe an assay to determine c-di-GMP degradation rates in vitro using 2′-O-(N′-methylanthraniloyl)-cyclic diguanylate (MANT-c-di-GMP). Additionally, a protocol for the production and purification of recombinant Pseudomonas aeruginosa RocR, a c-di-GMP-specific PDE that may serve as a control in MANT-c-di-GMP assays, is provided. The use of the fluorescent MANT-c-di-GMP analogue can deliver fundamental information about PDE function, and is suitable for identifying and investigating c-di-GMP-specific PDE activators and inhibitors.",
keywords = "Cyclic diguanylate, MANT-c-di-GMP, Phosphodiesterase, RocR, pGpG",
author = "Dorit Eli and Randall, \{Trevor E.\} and Henrik Almblad and Harrison, \{Joe J.\} and Ehud Banin",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-7240-1\_20",
language = "الإنجليزيّة",
series = "Methods in Molecular Biology",
pages = "263--278",
booktitle = "Methods in Molecular Biology",
}