Measles Virus Persistent Infection of Human Induced Pluripotent Stem Cells

Hila Naaman, Tatiana Rabinski, Avi Yizhak, Solly Mizrahi, Yonat Shemer Avni, Ran Taube, Bracha Rager, Yacov Weinstein, Glenn Rall, Jacob Gopas, Rivka Ofir

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles. Following MV persistent infection, BGU-iPSCs and EMF-iPSCs remain pluripotent and can differentiate in vitro into the three germ layers. This includes cells expressing the neuronal differentiation markers: NF68 and miRNA-124. Since the MV does not integrate into the cell's genome, it can be utilized as a vehicle to systematically introduce genes into iPSC, to dissect and to define factors regulating lineage differentiation.

Original languageAmerican English
Pages (from-to)17-26
Number of pages10
JournalCellular Reprogramming
Volume20
Issue number1
DOIs
StatePublished - 1 Feb 2018

Keywords

  • human-induced pluripotent stem cells
  • measles virus
  • persistent infection

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Developmental Biology
  • Cell Biology

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