MDC1 is ubiquitylated on its tandem BRCT domain and directly binds RAP80 in a UBC13-dependent manner

Carmit Strauss, Tomer Halevy, Michal Macarov, Liron Argaman, Michal Goldberg

Research output: Contribution to journalArticlepeer-review

Abstract

The cellular response to DNA damage is essential for maintenance of genomic stability. MDC1 is a key member of the DNA damage response. It is an adaptor protein that binds and recruits proteins to sites of DNA damage, a crucial step for a proper response. MDC1 contains several protein-protein interacting modules, including a tandem BRCT domain that mediates various interactions involving MDC1. Here we demonstrate that MDC1 binds directly to RAP80, which is a DNA damage response protein that recruits BRCA1 to sites of damage. The interaction between MDC1 and RAP80 requires the tandem BRCT domain of MDC1 and the ubiquitin-interacting motifs of RAP80. Moreover, the interaction depends on UBC13, an E2 ubiquitin ligase that catalyzes K63-linked poly-ubiquitin chain formation. The results highly propose that the interaction between MDC1 and RAP80 depends on a ubiquitylation event, which we found to take place on K-1977 of MDC1. This study provides the first evidence that interactions involving MDC1 can be regulated by ubiquitylation.

Original languageAmerican English
Pages (from-to)806-814
Number of pages9
JournalDNA Repair
Volume10
Issue number8
DOIs
StatePublished - 15 Aug 2011

Keywords

  • DNA double-strand break
  • MDC1
  • RAP80
  • The DNA damage response
  • Ubiquitin-interacting motif
  • Ubiquitylation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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