MBP-FGF2-Immobilized Matrix Maintains Self-Renewal and Myogenic Differentiation Potential of Skeletal Muscle Stem Cells

Jay Prakash Sah, Nguyen Thi Thu Hao, Yunhye Kim, Tamar Eigler, Eldad Tzahor, Sang-Heon Kim, Yongsung Hwang, Jeong Kyo Yoon

Research output: Contribution to journalArticlepeer-review

Abstract

The robust capacity of skeletal muscle stem cells (SkMSCs, or satellite cells) to regenerate into new muscles in vivo has offered promising therapeutic options for the treatment of degenerative muscle diseases. However, the practical use of SkMSCs to treat muscle diseases is limited, owing to their inability to expand in vitro under defined cultivation conditions without loss of engraftment efficiency. To develop an optimal cultivation condition for SkMSCs, we investigated the behavior of SkMSCs on synthetic maltose-binding protein (MBP)-fibro blast growth factor 2 (FGF2)-immobilized matrix in vitro. We found that the chemically well-defined, xeno-free MBP-FGF2-immobilized matrix effectively supports SkMSC growth without reducing their differentiation potential in vitro. Our data highlights the possible application of the MBP-FGF2 matrix for SkMSC expansion in vitro.

Original languageEnglish
Pages (from-to)360-366
Number of pages7
JournalInternational Journal of Stem Cells
Volume12
Issue number2
DOIs
StatePublished - 30 Jul 2019

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Developmental Biology

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