LuxR solos in the plant endophyte kosakonia sp. Strain KO348

Susan Mosquito; Xianfa Meng; Giulia Devescovi; Iris Bertani; Alexander M. Geller; Asaf Levy; Michael P. Myers; Cristina Bez; Sonia Covaceuszach; Vittorio Venturi

doi:https://doi.org/10.1128/AEM.00622-20

LuxR solos in the plant endophyte kosakonia sp. Strain KO348

Susan Mosquito, Xianfa Meng, Giulia Devescovi, Iris Bertani, Alexander M. Geller, Asaf Levy, Michael P. Myers, Cristina Bez, Sonia Covaceuszach, Vittorio Venturi

Department of Plant Pathology and Microbiology

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization.

Original language	English
Article number	e00622-20
Journal	Applied and Environmental Microbiology
Volume	86
Issue number	13
DOIs	https://doi.org/10.1128/AEM.00622-20
State	Published - 1 Jul 2020

Keywords

Bacteria
Cell-cell signaling
Endophyte
Gene regulation
LuxR solos

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

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https://doi.org/10.1128/AEM.00622-20

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title = "LuxR solos in the plant endophyte kosakonia sp. Strain KO348",

abstract = "Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization.",

keywords = "Bacteria, Cell-cell signaling, Endophyte, Gene regulation, LuxR solos",

author = "Susan Mosquito and Xianfa Meng and Giulia Devescovi and Iris Bertani and Geller, {Alexander M.} and Asaf Levy and Myers, {Michael P.} and Cristina Bez and Sonia Covaceuszach and Vittorio Venturi",

note = "Publisher Copyright: {\textcopyright} 2020 Mosquito et al.",

year = "2020",

month = jul,

day = "1",

doi = "https://doi.org/10.1128/AEM.00622-20",

language = "الإنجليزيّة",

volume = "86",

journal = "Applied and Environmental Microbiology",

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TY - JOUR

T1 - LuxR solos in the plant endophyte kosakonia sp. Strain KO348

AU - Mosquito, Susan

AU - Meng, Xianfa

AU - Devescovi, Giulia

AU - Bertani, Iris

AU - Geller, Alexander M.

AU - Levy, Asaf

AU - Myers, Michael P.

AU - Bez, Cristina

AU - Covaceuszach, Sonia

AU - Venturi, Vittorio

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization.

AB - Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization.

KW - Bacteria

KW - Cell-cell signaling

KW - Endophyte

KW - Gene regulation

KW - LuxR solos

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U2 - https://doi.org/10.1128/AEM.00622-20

DO - https://doi.org/10.1128/AEM.00622-20

M3 - مقالة

C2 - 32332134

SN - 0099-2240

VL - 86

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 13

M1 - e00622-20

ER -

LuxR solos in the plant endophyte kosakonia sp. Strain KO348

Abstract

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

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