TY - JOUR
T1 - Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice
AU - Ulivi, Alessandro F.
AU - Castello-VValdow, Tim P.
AU - Weston, Ghabiba
AU - Yan, Long
AU - Yasuda, Ryohei
AU - Chen, Alon
AU - Attardo, Alessio
N1 - U. A. F. is supported by the Schram foundation; C.-W. T. P. and W. G. are supported by the Max Planck Society; L.Y. and R.Y. are supported by the Max Planck Society and National Institute of Health (R01MH080047, 1DP1NS096787); A. C. is supported by an FP7 Grant from the European Research Council, the ERANET and I-CORE programs, the Chief Scientist Office of the Israeli Ministry of Health, the Federal Ministry of Education and Research, Roberto and Renata Ruhman, Bruno and Simone Lich, the Nella and Leon Benoziyo Center for Neurological Diseases, the Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics, The Israel Science Foundation the Perlman Family, Adelis, Marc Besen, Pratt and Irving I. Moskowitz foundations; A. A. is supported by the Max Planck Society, the Schram foundation and the Deutsche Forschungsgemeinschaft (DFG). The 3P images were acquired during the Advanced Course on Neuroimaging Techniques at the Max Planck Florida Institute for Neuroscience. The Advanced Course on Neuroimaging Techniques is supported by the Max Planck Society, the Florida State Max Planck Scientific Fellowship program and by the Max Planck Florida Institute Corporation Partnership program. We would like to thank Thorlabs, Coherent and SpectraPhysics for providing support and equipment for the 2P / 3P imaging system during the course. We are also grateful to Henry Haeberle and Melissa Eberle for assistance with the system during the course.
PY - 2019/6/19
Y1 - 2019/6/19
N2 - Two-photon microscopy is a fundamental tool for neuroscience as it permits investigation of the brain of live animals at spatial scales ranging from subcellular to network levels and at temporal scales from milliseconds to weeks. In addition, two-photon imaging can be combined with a variety of behavioral tasks to explore the causal relationships between brain function and behavior. However, in mammals, limited penetration and scattering of light have limited two-photon intravital imaging mostly to superficial brain regions, thus precluding longitudinal investigation of deep-brain areas such as the hippocampus. The hippocampus is involved in spatial navigation and episodic memory and is a long-standing model used to study cellular as well as cognitive processes important for learning and recall, both in health and disease. Here, a preparation that enables chronic optical access to the dorsal hippocampus in living mice is detailed. This preparation can be combined with two-photon optical imaging at cellular and subcellular resolution in head fixed, anesthetized live mice over several weeks. These techniques enable repeated imaging of neuronal structure or activity-evoked plasticity in tens to hundreds of neurons in the dorsal hippocampal CA1. Furthermore, this chronic preparation can be used in combination with other techniques such as micro-endoscopy, head-mounted wide field microscopy or three-photon microscopy, thus greatly expanding the toolbox to study cellular and network processes involved in learning and memory.
AB - Two-photon microscopy is a fundamental tool for neuroscience as it permits investigation of the brain of live animals at spatial scales ranging from subcellular to network levels and at temporal scales from milliseconds to weeks. In addition, two-photon imaging can be combined with a variety of behavioral tasks to explore the causal relationships between brain function and behavior. However, in mammals, limited penetration and scattering of light have limited two-photon intravital imaging mostly to superficial brain regions, thus precluding longitudinal investigation of deep-brain areas such as the hippocampus. The hippocampus is involved in spatial navigation and episodic memory and is a long-standing model used to study cellular as well as cognitive processes important for learning and recall, both in health and disease. Here, a preparation that enables chronic optical access to the dorsal hippocampus in living mice is detailed. This preparation can be combined with two-photon optical imaging at cellular and subcellular resolution in head fixed, anesthetized live mice over several weeks. These techniques enable repeated imaging of neuronal structure or activity-evoked plasticity in tens to hundreds of neurons in the dorsal hippocampal CA1. Furthermore, this chronic preparation can be used in combination with other techniques such as micro-endoscopy, head-mounted wide field microscopy or three-photon microscopy, thus greatly expanding the toolbox to study cellular and network processes involved in learning and memory.
U2 - 10.3791/59598
DO - 10.3791/59598
M3 - مقالة
SN - 1940-087X
VL - 2019
JO - Jove-Journal Of Visualized Experiments
JF - Jove-Journal Of Visualized Experiments
IS - 148
M1 - e59598
ER -