Live cell microRNA imaging using cascade hybridization reaction

Zoya Cheglakov; Timothy M. Cronin; Chuan He; Yossi Weizmann

doi:https://doi.org/10.1021/jacs.5b01451

Live cell microRNA imaging using cascade hybridization reaction

Zoya Cheglakov, Timothy M. Cronin, Chuan He, Yossi Weizmann

Research output: Contribution to journal › Article › peer-review

Abstract

Recent advances in RNA research have posed new directives in biology and chemistry to uncover the complex roles of ribonucleic acids in cellular processes. Innovative techniques to visualize native RNAs, particularly, short, low-abundance RNAs in live cells, can dramatically impact current research on the roles of RNAs in biology. Herein, we report a novel method for real-time, microRNA imaging inside live cells based on programmable oligonucleotide probes, which self-assemble through the Cascade Hybridization Reaction (CHR).

Original language	American English
Pages (from-to)	6116-6119
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	137
Issue number	19
DOIs	https://doi.org/10.1021/jacs.5b01451
State	Published - 20 May 2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
Biochemistry
Catalysis
Colloid and Surface Chemistry

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https://doi.org/10.1021/jacs.5b01451

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abstract = "Recent advances in RNA research have posed new directives in biology and chemistry to uncover the complex roles of ribonucleic acids in cellular processes. Innovative techniques to visualize native RNAs, particularly, short, low-abundance RNAs in live cells, can dramatically impact current research on the roles of RNAs in biology. Herein, we report a novel method for real-time, microRNA imaging inside live cells based on programmable oligonucleotide probes, which self-assemble through the Cascade Hybridization Reaction (CHR).",

author = "Zoya Cheglakov and Cronin, {Timothy M.} and Chuan He and Yossi Weizmann",

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AU - Cronin, Timothy M.

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AB - Recent advances in RNA research have posed new directives in biology and chemistry to uncover the complex roles of ribonucleic acids in cellular processes. Innovative techniques to visualize native RNAs, particularly, short, low-abundance RNAs in live cells, can dramatically impact current research on the roles of RNAs in biology. Herein, we report a novel method for real-time, microRNA imaging inside live cells based on programmable oligonucleotide probes, which self-assemble through the Cascade Hybridization Reaction (CHR).

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JF - Journal of the American Chemical Society

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Live cell microRNA imaging using cascade hybridization reaction

Abstract

All Science Journal Classification (ASJC) codes

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