@inbook{3f304b4282264b3683926ef51e99da3e,
title = "LeEIX2 interactors{\textquoteright} analysis and EIX-mediated responses measurement",
abstract = "Plant-pathogen interactions involve a large number of wide regulatory systems, necessary for plant defense responses against pathogen attack. The fungal protein ethylene-inducing xylanase (EIX) elicits defense responses in specific cultivars of tobacco and tomato. The response to EIX is controlled by a single locus encoding for LeEIX2, a leucine-rich-repeat receptor-like-protein (LRR-RLP). As an RLP, LeEIX2 does not possess an obvious cytoplasmic signaling moiety such as a kinase domain. To study LeEIX2 mode of action, it is essential to identify the potential interactors involved after EIX perception. Here, we describe the in vivo co-IP methodology used for protein interaction verification and ethylene and ROS (reactive oxygen species) measurements used for physiological effects assessment.",
keywords = "Co-IP, Defense responses, Ethylene-inducing xylanase, LRR-RLP, MAMP, ROS",
author = "Meirav Leibman-Markus and Silvia Schuster and Adi Avni",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6859-6_13",
language = "الإنجليزيّة",
series = "Methods in Molecular Biology",
pages = "167--172",
booktitle = "Methods in Molecular Biology",
}