Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments

Amit Tzur; Meirav Noach; Hadas Nevenzal; Yair Glick; Evelin Chorni; Dorit Avrahami; Efrat Barbiro-Michaely; Doron Gerber

doi:https://doi.org/10.1096/fasebj.29.1_supplement.884.38

Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments

Amit Tzur, Meirav Noach, Hadas Nevenzal, Yair Glick, Evelin Chorni, Dorit Avrahami, Efrat Barbiro-Michaely, Doron Gerber

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.

Original language	English
Pages (from-to)	884.38
Journal	FASEB Journal
Volume	29
Issue number	S1
DOIs	https://doi.org/10.1096/fasebj.29.1_supplement.884.38
State	Published - 1 Apr 2015

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abstract = "We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.",

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AU - Tzur, Amit

AU - Noach, Meirav

AU - Nevenzal, Hadas

AU - Glick, Yair

AU - Chorni, Evelin

AU - Avrahami, Dorit

AU - Barbiro-Michaely, Efrat

AU - Gerber, Doron

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PY - 2015/4/1

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N2 - We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.

AB - We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.

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M3 - مقالة

SN - 0892-6638

VL - 29

SP - 884.38

JO - FASEB Journal

JF - FASEB Journal

IS - S1

ER -

Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments

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