Large-scale automated histology in the pursuit of Connectomes

David Kleinfeld; Arjun Bharioke; Pablo Blinder; Davi D. Bock; Kevin L. Briggman; Dmitri B. Chklovskii; Winfried Denk; Moritz Helmstaedter; John P. Kaufhold; Wei Chung Allen Lee; Hanno S. Meyer; Kristina D. Micheva; Marcel Oberlaender; Steffen Prohaska; R. Clay Reid; Stephen J. Smith; Shinya Takemura; Philbert S. Tsai; Bert Sakmann

doi:https://doi.org/10.1523/JNEUROSCI.4077-11.2011

Large-scale automated histology in the pursuit of Connectomes

David Kleinfeld, Arjun Bharioke, Pablo Blinder, Davi D. Bock, Kevin L. Briggman, Dmitri B. Chklovskii, Winfried Denk, Moritz Helmstaedter, John P. Kaufhold, Wei Chung Allen Lee, Hanno S. Meyer, Kristina D. Micheva, Marcel Oberlaender, Steffen Prohaska, R. Clay Reid, Stephen J. Smith, Shinya Takemura, Philbert S. Tsai, Bert Sakmann

Research output: Contribution to journal › Article › peer-review

Abstract

How does the brain compute? Answering this question necessitates neuronal connectomes, annotated graphs of all synaptic connections within defined brain areas. Further, understanding the energetics of the brain's computations requires vascular graphs. The assembly of a connectome requires sensitive hardware tools to measure neuronal and neurovascular features in all three dimensions, as well as software and machine learning for data analysis and visualization. We present the state of the art on the reconstruction of circuits and vasculature that link brain anatomy and function. Analysis at the scale of tens of nanometers yields connections between identified neurons, while analysis at the micrometer scale yields probabilistic rules of connection between neurons and exact vascular connectivity.

Original language	English
Pages (from-to)	16125-16138
Number of pages	14
Journal	Journal of Neuroscience
Volume	31
Issue number	45
DOIs	https://doi.org/10.1523/JNEUROSCI.4077-11.2011
State	Published - 9 Nov 2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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https://doi.org/10.1523/JNEUROSCI.4077-11.2011

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Kleinfeld, D., Bharioke, A., Blinder, P., Bock, D. D., Briggman, K. L., Chklovskii, D. B., Denk, W., Helmstaedter, M., Kaufhold, J. P., Lee, W. C. A., Meyer, H. S., Micheva, K. D., Oberlaender, M., Prohaska, S., Reid, R. C., Smith, S. J., Takemura, S., Tsai, P. S., & Sakmann, B. (2011). Large-scale automated histology in the pursuit of Connectomes. Journal of Neuroscience, 31(45), 16125-16138. https://doi.org/10.1523/JNEUROSCI.4077-11.2011

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title = "Large-scale automated histology in the pursuit of Connectomes",

abstract = "How does the brain compute? Answering this question necessitates neuronal connectomes, annotated graphs of all synaptic connections within defined brain areas. Further, understanding the energetics of the brain's computations requires vascular graphs. The assembly of a connectome requires sensitive hardware tools to measure neuronal and neurovascular features in all three dimensions, as well as software and machine learning for data analysis and visualization. We present the state of the art on the reconstruction of circuits and vasculature that link brain anatomy and function. Analysis at the scale of tens of nanometers yields connections between identified neurons, while analysis at the micrometer scale yields probabilistic rules of connection between neurons and exact vascular connectivity.",

author = "David Kleinfeld and Arjun Bharioke and Pablo Blinder and Bock, {Davi D.} and Briggman, {Kevin L.} and Chklovskii, {Dmitri B.} and Winfried Denk and Moritz Helmstaedter and Kaufhold, {John P.} and Lee, {Wei Chung Allen} and Meyer, {Hanno S.} and Micheva, {Kristina D.} and Marcel Oberlaender and Steffen Prohaska and Reid, {R. Clay} and Smith, {Stephen J.} and Shinya Takemura and Tsai, {Philbert S.} and Bert Sakmann",

year = "2011",

month = nov,

day = "9",

doi = "https://doi.org/10.1523/JNEUROSCI.4077-11.2011",

language = "الإنجليزيّة",

volume = "31",

pages = "16125--16138",

journal = "Journal of Neuroscience",

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}

Kleinfeld, D, Bharioke, A, Blinder, P, Bock, DD, Briggman, KL, Chklovskii, DB, Denk, W, Helmstaedter, M, Kaufhold, JP, Lee, WCA, Meyer, HS, Micheva, KD, Oberlaender, M, Prohaska, S, Reid, RC, Smith, SJ, Takemura, S, Tsai, PS & Sakmann, B 2011, 'Large-scale automated histology in the pursuit of Connectomes', Journal of Neuroscience, vol. 31, no. 45, pp. 16125-16138. https://doi.org/10.1523/JNEUROSCI.4077-11.2011

TY - JOUR

T1 - Large-scale automated histology in the pursuit of Connectomes

AU - Kleinfeld, David

AU - Bharioke, Arjun

AU - Blinder, Pablo

AU - Bock, Davi D.

AU - Briggman, Kevin L.

AU - Chklovskii, Dmitri B.

AU - Denk, Winfried

AU - Helmstaedter, Moritz

AU - Kaufhold, John P.

AU - Lee, Wei Chung Allen

AU - Meyer, Hanno S.

AU - Micheva, Kristina D.

AU - Oberlaender, Marcel

AU - Prohaska, Steffen

AU - Reid, R. Clay

AU - Smith, Stephen J.

AU - Takemura, Shinya

AU - Tsai, Philbert S.

AU - Sakmann, Bert

PY - 2011/11/9

Y1 - 2011/11/9

N2 - How does the brain compute? Answering this question necessitates neuronal connectomes, annotated graphs of all synaptic connections within defined brain areas. Further, understanding the energetics of the brain's computations requires vascular graphs. The assembly of a connectome requires sensitive hardware tools to measure neuronal and neurovascular features in all three dimensions, as well as software and machine learning for data analysis and visualization. We present the state of the art on the reconstruction of circuits and vasculature that link brain anatomy and function. Analysis at the scale of tens of nanometers yields connections between identified neurons, while analysis at the micrometer scale yields probabilistic rules of connection between neurons and exact vascular connectivity.

AB - How does the brain compute? Answering this question necessitates neuronal connectomes, annotated graphs of all synaptic connections within defined brain areas. Further, understanding the energetics of the brain's computations requires vascular graphs. The assembly of a connectome requires sensitive hardware tools to measure neuronal and neurovascular features in all three dimensions, as well as software and machine learning for data analysis and visualization. We present the state of the art on the reconstruction of circuits and vasculature that link brain anatomy and function. Analysis at the scale of tens of nanometers yields connections between identified neurons, while analysis at the micrometer scale yields probabilistic rules of connection between neurons and exact vascular connectivity.

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DO - https://doi.org/10.1523/JNEUROSCI.4077-11.2011

M3 - مقالة

C2 - 22072665

SN - 0270-6474

VL - 31

SP - 16125

EP - 16138

JO - Journal of Neuroscience

JF - Journal of Neuroscience

IS - 45

ER -

Large-scale automated histology in the pursuit of Connectomes

Abstract

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