TY - JOUR
T1 - Laboratory evaluation of the ESwab transport system for the recovery of carbapenem-resistant Acinetobacter baumannii
AU - Moran-Gilad, J.
AU - Navon-Venezia, S.
AU - Carmeli, Y.
N1 - Funding Information: Acknowledgements This work was supported in part by the European Commission Grant (FP6): European Network for Mastering Hospital Antimicrobial Resistance and its Spread into the Community (MOSAR; LSHPCT-2007–037941).
PY - 2012/7/1
Y1 - 2012/7/1
N2 - Microbiological surveillance for detection of carbapenem-resistant A. baumannii is important, but recovery of A. baumannii is inadequate. We studied A. baumannii recovery by a particular transport system that is possibly superior over standard swabs, using reference and clinical strains. First, the recovery rates relating to the various swabs were compared with regard to various combinations of transport times (0 h, 1 h, 24 h, 48 h), storage times (0 weeks, 1 week, 2 weeks, 4 weeks) and storage temperatures (4°c,-80°c) using live counts. Second, the recovery of different inocula of strains mixed with fecal microbiota was evaluated by plating on selective medium. The new transport system exhibited a decline of >3log10 under almost all conditions studied and performed better than standard swabs under several conditions. If plated on selective media, the new transport system performed well, even after prolonged transport or with a low inoculum, and its processing could be delayed by up to 2 weeks, especially if refrigerated. The new transport system may thus enhance A. baumannii surveillance.
AB - Microbiological surveillance for detection of carbapenem-resistant A. baumannii is important, but recovery of A. baumannii is inadequate. We studied A. baumannii recovery by a particular transport system that is possibly superior over standard swabs, using reference and clinical strains. First, the recovery rates relating to the various swabs were compared with regard to various combinations of transport times (0 h, 1 h, 24 h, 48 h), storage times (0 weeks, 1 week, 2 weeks, 4 weeks) and storage temperatures (4°c,-80°c) using live counts. Second, the recovery of different inocula of strains mixed with fecal microbiota was evaluated by plating on selective medium. The new transport system exhibited a decline of >3log10 under almost all conditions studied and performed better than standard swabs under several conditions. If plated on selective media, the new transport system performed well, even after prolonged transport or with a low inoculum, and its processing could be delayed by up to 2 weeks, especially if refrigerated. The new transport system may thus enhance A. baumannii surveillance.
UR - http://www.scopus.com/inward/record.url?scp=84865585853&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/s10096-011-1460-2
DO - https://doi.org/10.1007/s10096-011-1460-2
M3 - Article
C2 - 22068274
SN - 0934-9723
VL - 31
SP - 1429
EP - 1433
JO - European Journal of Clinical Microbiology and Infectious Diseases
JF - European Journal of Clinical Microbiology and Infectious Diseases
IS - 7
ER -