Label-free optical-resolution photoacoustic endomicroscopy in vivo

Joon Mo Yang, Chiye Li, Ruimin Chen, Bin Rao, Junjie Yao, Cheng Hung Yeh, Amos Danielli, Konstantin Maslov, Qifa Zhou, K. Kirk Shung, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an optical-resolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ∼10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2015
EditorsAlexander A. Oraevsky, Lihong V. Wang
ISBN (Electronic)9781628414134
StatePublished - 11 Mar 2015
Externally publishedYes
EventPhotons Plus Ultrasound: Imaging and Sensing 2015 - San Francisco, United States
Duration: 8 Feb 201510 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2015
Country/TerritoryUnited States
CitySan Francisco


  • In vivo imaging
  • Intravital microscopy
  • Label-free imaging
  • Optical-resolution
  • Photoacoustic endomicroscopy
  • Photoacoustic endoscopy
  • Photoacoustic microscopy
  • Rat colorectum

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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