Label-free optical monitoring of proteolytic reaction products using nanoporous silica colloidal assembly

Giorgi Shtenberg; Naama Massad-Ivanir; Amir Khabibullin; Ilya Zharov; Ester Segal

doi:10.1016/j.snb.2018.02.057

Label-free optical monitoring of proteolytic reaction products using nanoporous silica colloidal assembly

Giorgi Shtenberg, Naama Massad-Ivanir, Amir Khabibullin, Ilya Zharov, Ester Segal

Technion - Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

An optical biosensor for rapid monitoring of proteolytic activity is constructed by immobilization of proteases onto multifunctional silica colloidal assembly (SCA). The SCA serves as Fabry-Pérot thin film, which is highly sensitive to the presence of biomolecules (e.g., enzymes, proteins and short peptides) within the nanostructure. Moreover, the SCA acts as a size-exclusion element, allowing to entrap and to concentrate the enzymatic degradation products for downstream mass spectrometry analysis for substrate profiling and cleavage sites identification.

Original language	English
Pages (from-to)	796-800
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	262
DOIs	https://doi.org/10.1016/j.snb.2018.02.057
State	Published - 1 Jun 2018

Keywords

Biosensors
Colloidal assembly
Enzyme
Interferometer
Mass spectrometry
Protease
Silica

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2018.02.057

Cite this

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title = "Label-free optical monitoring of proteolytic reaction products using nanoporous silica colloidal assembly",

abstract = "An optical biosensor for rapid monitoring of proteolytic activity is constructed by immobilization of proteases onto multifunctional silica colloidal assembly (SCA). The SCA serves as Fabry-P{\'e}rot thin film, which is highly sensitive to the presence of biomolecules (e.g., enzymes, proteins and short peptides) within the nanostructure. Moreover, the SCA acts as a size-exclusion element, allowing to entrap and to concentrate the enzymatic degradation products for downstream mass spectrometry analysis for substrate profiling and cleavage sites identification.",

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author = "Giorgi Shtenberg and Naama Massad-Ivanir and Amir Khabibullin and Ilya Zharov and Ester Segal",

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doi = "10.1016/j.snb.2018.02.057",

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AU - Shtenberg, Giorgi

AU - Massad-Ivanir, Naama

AU - Khabibullin, Amir

AU - Zharov, Ilya

AU - Segal, Ester

PY - 2018/6/1

Y1 - 2018/6/1

N2 - An optical biosensor for rapid monitoring of proteolytic activity is constructed by immobilization of proteases onto multifunctional silica colloidal assembly (SCA). The SCA serves as Fabry-Pérot thin film, which is highly sensitive to the presence of biomolecules (e.g., enzymes, proteins and short peptides) within the nanostructure. Moreover, the SCA acts as a size-exclusion element, allowing to entrap and to concentrate the enzymatic degradation products for downstream mass spectrometry analysis for substrate profiling and cleavage sites identification.

AB - An optical biosensor for rapid monitoring of proteolytic activity is constructed by immobilization of proteases onto multifunctional silica colloidal assembly (SCA). The SCA serves as Fabry-Pérot thin film, which is highly sensitive to the presence of biomolecules (e.g., enzymes, proteins and short peptides) within the nanostructure. Moreover, the SCA acts as a size-exclusion element, allowing to entrap and to concentrate the enzymatic degradation products for downstream mass spectrometry analysis for substrate profiling and cleavage sites identification.

KW - Biosensors

KW - Colloidal assembly

KW - Enzyme

KW - Interferometer

KW - Mass spectrometry

KW - Protease

KW - Silica

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DO - 10.1016/j.snb.2018.02.057

M3 - مقالة

SN - 0925-4005

VL - 262

SP - 796

EP - 800

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Label-free optical monitoring of proteolytic reaction products using nanoporous silica colloidal assembly

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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