Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Stefan Jaric; Aabha Bajaj; Vladimir Vukic; Ivana Gadjanski; Ibrahim Abdulhalim; Ivan Bobrinetskiy

doi:https://doi.org/10.3390/toxins15050326

Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Stefan Jaric, Aabha Bajaj, Vladimir Vukic, Ivana Gadjanski, Ibrahim Abdulhalim, Ivan Bobrinetskiy

Research output: Contribution to journal › Article › peer-review

Abstract

In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer’s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.

Original language	American English
Article number	326
Journal	Toxins
Volume	15
Issue number	5
DOIs	https://doi.org/10.3390/toxins15050326
State	Published - 1 May 2023

Keywords

CVD graphene
aptamer
cyanotoxins
label-free biosensing
surface plasmon resonance

All Science Journal Classification (ASJC) codes

Health, Toxicology and Mutagenesis
Toxicology

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https://doi.org/10.3390/toxins15050326

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title = "Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor",

abstract = "In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer{\textquoteright}s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.",

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AU - Jaric, Stefan

AU - Bajaj, Aabha

AU - Vukic, Vladimir

AU - Gadjanski, Ivana

AU - Abdulhalim, Ibrahim

AU - Bobrinetskiy, Ivan

PY - 2023/5/1

Y1 - 2023/5/1

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AB - In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer’s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.

KW - CVD graphene

KW - aptamer

KW - cyanotoxins

KW - label-free biosensing

KW - surface plasmon resonance

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JO - Toxins

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Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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