Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Stefan Jaric; Aabha Bajaj; Vladimir Vukic; Ivana Gadjanski; Ibrahim Abdulhalim; Ivan Bobrinetskiy

doi:https://doi.org/10.3390/toxins15050326

Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Stefan Jaric, Aabha Bajaj, Vladimir Vukic, Ivana Gadjanski, Ibrahim Abdulhalim, Ivan Bobrinetskiy

Unit of Photonics and Electro-Optics Engineering

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer’s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.

Original language	English
Article number	326
Journal	Toxins
Volume	15
Issue number	5
DOIs	https://doi.org/10.3390/toxins15050326
State	Published - 1 May 2023

Keywords

CVD graphene
aptamer
cyanotoxins
label-free biosensing
surface plasmon resonance

All Science Journal Classification (ASJC) codes

Health, Toxicology and Mutagenesis
Toxicology

Access to Document

https://doi.org/10.3390/toxins15050326

Cite this

@article{35ebdfa0a78a477784f80fa0215cae3d,

title = "Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor",

abstract = "In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer{\textquoteright}s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.",

keywords = "CVD graphene, aptamer, cyanotoxins, label-free biosensing, surface plasmon resonance",

author = "Stefan Jaric and Aabha Bajaj and Vladimir Vukic and Ivana Gadjanski and Ibrahim Abdulhalim and Ivan Bobrinetskiy",

note = "Funding Information: This research was funded by projects funded by the European Union{\textquoteright}s Horizon 2020 research and innovation programme: the IPANEMA, under grant agreement No. 872662 (https://doi.org/10.3030/872662, accessed on 21 March 2023) and work was supported in part by ANTARES, under grant agreement No. 664387 (https://doi.org/10.3030/739570, accessed on 21 March 2023), and by the Ministry of Science, Technological Development, and Innovations of the Republic of Serbia (grant numbers 451-03-47/2023-01/200134 and number 451-03-47/2023-01/200358). Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = may,

day = "1",

doi = "https://doi.org/10.3390/toxins15050326",

language = "English",

volume = "15",

journal = "Toxins",

issn = "2072-6651",

publisher = "MDPI Multidisciplinary Digital Publishing Institute",

number = "5",

}

TY - JOUR

T1 - Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

AU - Jaric, Stefan

AU - Bajaj, Aabha

AU - Vukic, Vladimir

AU - Gadjanski, Ivana

AU - Abdulhalim, Ibrahim

AU - Bobrinetskiy, Ivan

N1 - Funding Information: This research was funded by projects funded by the European Union’s Horizon 2020 research and innovation programme: the IPANEMA, under grant agreement No. 872662 (https://doi.org/10.3030/872662, accessed on 21 March 2023) and work was supported in part by ANTARES, under grant agreement No. 664387 (https://doi.org/10.3030/739570, accessed on 21 March 2023), and by the Ministry of Science, Technological Development, and Innovations of the Republic of Serbia (grant numbers 451-03-47/2023-01/200134 and number 451-03-47/2023-01/200358). Publisher Copyright: © 2023 by the authors.

PY - 2023/5/1

Y1 - 2023/5/1

N2 - In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer’s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.

AB - In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer’s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.

KW - CVD graphene

KW - aptamer

KW - cyanotoxins

KW - label-free biosensing

KW - surface plasmon resonance

UR - http://www.scopus.com/inward/record.url?scp=85160341163&partnerID=8YFLogxK

U2 - https://doi.org/10.3390/toxins15050326

DO - https://doi.org/10.3390/toxins15050326

M3 - Article

C2 - 37235360

SN - 2072-6651

VL - 15

JO - Toxins

JF - Toxins

IS - 5

M1 - 326

ER -

Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Abstract

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this