ITIA: Tripling the field-of-view in off-axis interferometric phase microscopy

Irena Frenklach, Pinhas Girshovitz, Natan T. Shaked

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Interferometric phase microscopy (IPM) is a quantitative optical imaging method for capturing the phase profiles of thin samples. While being an invaluable tool for biological and medical research, most IPM setups are unfriendly for inexperienced users, and have limited field of view (FOV). To overcome the limited FOV problem, it is possible to scan the sample and record a wider FOV. However, dynamic samples might move by the time the scan is over. Here, we review our previously published work presenting a new quantitative imaging technique, referred to as interferometry with tripled-imaging area (ITIA), which is capable of capturing three off-axis interferometric fields of view in a single camera exposure, thus tripling the acquired information without the need to scan the sample, without decreasing the image resolution, and without changing the system magnification. Our experimental demonstrations were done by using an inverted transmission microscope illuminated by a Helium-Neon laser. The sample is projected onto the image plane at the output of the microscope, where the ITIA module is placed. Various biological and non-biological samples were imaged.

Original languageEnglish
Title of host publicationInterferometry XVII
Subtitle of host publicationTechniques and Analysis
EditorsKatherine Creath, Jan Burke, Joanna Schmit
PublisherSPIE
ISBN (Electronic)9781628412307
DOIs
StatePublished - 1 Jan 2014
EventInterferometry XVII: Techniques and Analysis - San Diego, United States
Duration: 17 Aug 201419 Aug 2014

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume9203

Conference

ConferenceInterferometry XVII: Techniques and Analysis
Country/TerritoryUnited States
CitySan Diego
Period17/08/1419/08/14

Keywords

  • Biological cell imaging
  • Holography
  • Interferometric Imaging
  • Interferometry
  • Multiplexing
  • Phase microscopy

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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