Isotachophoresis-based surface immunoassay

F. Paratore, T. Zeidman Kalman, T. Rosenfeld, G. V. Kaigala, Moran Bercovici

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Surface immunoassays (e.g. ELISA, lateral flow) are the gold standard in clinical and point-of-care diagnostics. In contrast to detection of nucleic acid sequences, where amplification methods can be used, detection of proteins must rely solely on the initial concentration of targets in the sample. Therefore, at low target concentrations, immunoassays are limited by the slow binding kinetics between targets and antibodies. We here demonstrate the use of isotachophoretic focusing for acceleration of antibody-protein binding kinetics, improving the limit of detection (LoD) by 50 to 100 fold as compared to standard immunoassays.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Pages665-666
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period9/10/1613/10/16

Keywords

  • Biosensor
  • Immunoassay
  • Isotachophoresis
  • Point-of-care diagnostics

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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