Abstract
The immune fraction of the tumor microenvironment has been proven to play a fundamental role in glioblastoma progression and therapeutic response. Here, we present a detailed magnetic-bead-enrichment-based protocol to isolate and analyze the composition of this fraction from mouse brain tumors. The protocol is optimized to achieve high yields of viable immune cells. We also detail characterization of the immune subtypes by FACS analysis. Our procedure is applicable for either lentiviral-induced tumors or transplant models in syngeneic immunocompetent mice. For complete details on the use and execution of this protocol, please refer to Magod et al. (2021).
| Original language | English |
|---|---|
| Article number | 101106 |
| Journal | STAR Protocols |
| Volume | 3 |
| Issue number | 1 |
| DOIs | |
| State | Published - 18 Mar 2022 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
-
SDG 3 Good Health and Well-being
Keywords
- Cancer
- Cell Biology
- Cell isolation
- Flow Cytometry/Mass Cytometry
- Immunology
- Model Organisms
- Neuroscience
All Science Journal Classification (ASJC) codes
- General Biochemistry,Genetics and Molecular Biology
- General Neuroscience
- General Immunology and Microbiology
- General Medicine
Fingerprint
Dive into the research topics of 'Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver