Is it necessary to wait several minutes between applications of different topical ophthalmic solutions? A preliminary study with tropicamide eye drops in healthy dogs

Objective: To evaluate the efficacy of topical tropicamide when placed at different time intervals before or after a saline drop. Animals studied: Eight healthy Labrador and golden retriever dogs. Procedures: The effect of 1% tropicamide on pupillary diameter (PD) was measured over 240 min when administered alone (control) and then 1 and 5 min prior to, or following, application of a saline drop, with 1-week washout between each of the five trials. Data were analyzed using repeated-measures ANOVA and Tukey post hoc test. Results: Only 6/110 pairwise comparisons among the 5 trials were statistically significant (p ≤.035), with post-hoc analysis showing no significant differences (p ≥.14) between the overall means of all trials. In all five trials, maximal PD was reached 30 min after tropicamide application and maintained until 210 min for 180 min (p =.0005). Conclusions: Our results suggest that waiting 1 min between applications of different ophthalmic solutions may be sufficient for maximal drug effect. Care should be taken when extrapolating these results to other species and different ophthalmic formulations.