Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire

Kenta Shinoda; Yaakov Maman; Andres Canela; David G. Schatz; Ferenc Livak; André Nussenzweig

doi:10.1016/j.celrep.2019.11.088

Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire

Kenta Shinoda, Yaakov Maman, Andres Canela, David G. Schatz, Ferenc Livak, André Nussenzweig

Bar-Ilan University - Azrieli Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Shinoda et al. demonstrate previously unknown, frequent RAG-dependent recombination within the Ig Vκ gene cluster, which is mediated by evolutionarily conserved flipped RSS embedded in bona fide RSS. These intra-Vκ cluster recombination events alter the available Vκ gene pool by deleting Vκ segments and shape the Vκ-Jκ immune repertoire.

Original language	English
Pages (from-to)	4471-4481.e6
Journal	Cell Reports
Volume	29
Issue number	13
DOIs	https://doi.org/10.1016/j.celrep.2019.11.088
State	Published - 24 Dec 2019

Keywords

END-seq
HTGTS
RAG
VDJ recombination
ig kappa locus
ig repertoire
immunoglobulin
recombination center
recombination signal sequence
variable

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

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10.1016/j.celrep.2019.11.088

Cite this

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title = "Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire",

abstract = "Shinoda et al. demonstrate previously unknown, frequent RAG-dependent recombination within the Ig Vκ gene cluster, which is mediated by evolutionarily conserved flipped RSS embedded in bona fide RSS. These intra-Vκ cluster recombination events alter the available Vκ gene pool by deleting Vκ segments and shape the Vκ-Jκ immune repertoire.",

keywords = "END-seq, HTGTS, RAG, VDJ recombination, ig kappa locus, ig repertoire, immunoglobulin, recombination center, recombination signal sequence, variable",

author = "Kenta Shinoda and Yaakov Maman and Andres Canela and Schatz, \{David G.\} and Ferenc Livak and Andr{\'e} Nussenzweig",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s)",

year = "2019",

month = dec,

day = "24",

doi = "10.1016/j.celrep.2019.11.088",

language = "الإنجليزيّة",

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pages = "4471--4481.e6",

journal = "Cell Reports",

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TY - JOUR

T1 - Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire

AU - Shinoda, Kenta

AU - Maman, Yaakov

AU - Canela, Andres

AU - Schatz, David G.

AU - Livak, Ferenc

AU - Nussenzweig, André

PY - 2019/12/24

Y1 - 2019/12/24

N2 - Shinoda et al. demonstrate previously unknown, frequent RAG-dependent recombination within the Ig Vκ gene cluster, which is mediated by evolutionarily conserved flipped RSS embedded in bona fide RSS. These intra-Vκ cluster recombination events alter the available Vκ gene pool by deleting Vκ segments and shape the Vκ-Jκ immune repertoire.

AB - Shinoda et al. demonstrate previously unknown, frequent RAG-dependent recombination within the Ig Vκ gene cluster, which is mediated by evolutionarily conserved flipped RSS embedded in bona fide RSS. These intra-Vκ cluster recombination events alter the available Vκ gene pool by deleting Vκ segments and shape the Vκ-Jκ immune repertoire.

KW - END-seq

KW - HTGTS

KW - RAG

KW - VDJ recombination

KW - ig kappa locus

KW - ig repertoire

KW - immunoglobulin

KW - recombination center

KW - recombination signal sequence

KW - variable

UR - http://www.scopus.com/inward/record.url?scp=85076757282&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2019.11.088

DO - 10.1016/j.celrep.2019.11.088

M3 - مقالة

C2 - 31875554

SN - 2211-1247

VL - 29

SP - 4471-4481.e6

JO - Cell Reports

JF - Cell Reports

IS - 13

ER -

Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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