Intra-individual diversity of bacteriophage susceptibility in Burkholderia cultured from cystic fibrosis sputum

Ortal Yerushalmy; Abby M. Korn; Guichun Yao; Ronen Hazan; Carlos F. Gonzalez; Jason J. Gill; Linda M. Kalikin; Theodore Spilker; Lindsay J. Caverly; Amy A. Bumford; Nathan R. Wallace; Saima Aslam; Ran Nir-Paz; Daria Van Tyne; John J. LiPuma

doi:10.1016/j.jcf.2025.05.008

Intra-individual diversity of bacteriophage susceptibility in Burkholderia cultured from cystic fibrosis sputum

Ortal Yerushalmy, Abby M. Korn, Guichun Yao, Ronen Hazan, Carlos F. Gonzalez, Jason J. Gill, Linda M. Kalikin, Theodore Spilker, Lindsay J. Caverly, Amy A. Bumford, Nathan R. Wallace, Saima Aslam, Ran Nir-Paz, Daria Van Tyne, John J. LiPuma

Institute of Biomedical Research at the Faculty of Dental Medicine

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

The use of bacteriophage (phage) to treat bacterial infection of airways in persons with cystic fibrosis (CF) is gaining interest. However, phenotypic diversification of bacteria during chronic airway infection presents a potential challenge to this therapy. We recovered and subcultured two or three Burkholderia colonies from each of 12 CF sputum samples. All isolates were tested for their susceptibility to a panel of 65 Burkholderia-targeting phages. We observed that 9 (75%) of the 12 colony sets comprised mixtures of isolates that were sensitive or resistant to one or more of the phages tested. The occurrence of mixed populations of phage-sensitive and phage-resistant Burkholderia in individuals with CF needs to be considered in the development of phage therapy for this patient population.

Original language	English
Journal	Journal of Cystic Fibrosis
DOIs	https://doi.org/10.1016/j.jcf.2025.05.008
State	Accepted/In press - 2025

Keywords

Bacteriophage therapy
Burkholderia airway infection
Cystic fibrosis

All Science Journal Classification (ASJC) codes

Pediatrics, Perinatology, and Child Health
Pulmonary and Respiratory Medicine

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10.1016/j.jcf.2025.05.008

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Yerushalmy, O., Korn, A. M., Yao, G., Hazan, R., Gonzalez, C. F., Gill, J. J., Kalikin, L. M., Spilker, T., Caverly, L. J., Bumford, A. A., Wallace, N. R., Aslam, S., Nir-Paz, R., Van Tyne, D., & LiPuma, J. J. (Accepted/In press). Intra-individual diversity of bacteriophage susceptibility in Burkholderia cultured from cystic fibrosis sputum. Journal of Cystic Fibrosis. https://doi.org/10.1016/j.jcf.2025.05.008

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title = "Intra-individual diversity of bacteriophage susceptibility in Burkholderia cultured from cystic fibrosis sputum",

abstract = "The use of bacteriophage (phage) to treat bacterial infection of airways in persons with cystic fibrosis (CF) is gaining interest. However, phenotypic diversification of bacteria during chronic airway infection presents a potential challenge to this therapy. We recovered and subcultured two or three Burkholderia colonies from each of 12 CF sputum samples. All isolates were tested for their susceptibility to a panel of 65 Burkholderia-targeting phages. We observed that 9 (75%) of the 12 colony sets comprised mixtures of isolates that were sensitive or resistant to one or more of the phages tested. The occurrence of mixed populations of phage-sensitive and phage-resistant Burkholderia in individuals with CF needs to be considered in the development of phage therapy for this patient population.",

keywords = "Bacteriophage therapy, Burkholderia airway infection, Cystic fibrosis",

author = "Ortal Yerushalmy and Korn, {Abby M.} and Guichun Yao and Ronen Hazan and Gonzalez, {Carlos F.} and Gill, {Jason J.} and Kalikin, {Linda M.} and Theodore Spilker and Caverly, {Lindsay J.} and Bumford, {Amy A.} and Wallace, {Nathan R.} and Saima Aslam and Ran Nir-Paz and {Van Tyne}, Daria and LiPuma, {John J.}",

note = "Publisher Copyright: {\textcopyright} 2025",

year = "2025",

doi = "10.1016/j.jcf.2025.05.008",

language = "الإنجليزيّة",

journal = "Journal of Cystic Fibrosis",

issn = "1569-1993",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Intra-individual diversity of bacteriophage susceptibility in Burkholderia cultured from cystic fibrosis sputum

AU - Yerushalmy, Ortal

AU - Korn, Abby M.

AU - Yao, Guichun

AU - Hazan, Ronen

AU - Gonzalez, Carlos F.

AU - Gill, Jason J.

AU - Kalikin, Linda M.

AU - Spilker, Theodore

AU - Caverly, Lindsay J.

AU - Bumford, Amy A.

AU - Wallace, Nathan R.

AU - Aslam, Saima

AU - Nir-Paz, Ran

AU - Van Tyne, Daria

AU - LiPuma, John J.

PY - 2025

Y1 - 2025

N2 - The use of bacteriophage (phage) to treat bacterial infection of airways in persons with cystic fibrosis (CF) is gaining interest. However, phenotypic diversification of bacteria during chronic airway infection presents a potential challenge to this therapy. We recovered and subcultured two or three Burkholderia colonies from each of 12 CF sputum samples. All isolates were tested for their susceptibility to a panel of 65 Burkholderia-targeting phages. We observed that 9 (75%) of the 12 colony sets comprised mixtures of isolates that were sensitive or resistant to one or more of the phages tested. The occurrence of mixed populations of phage-sensitive and phage-resistant Burkholderia in individuals with CF needs to be considered in the development of phage therapy for this patient population.

AB - The use of bacteriophage (phage) to treat bacterial infection of airways in persons with cystic fibrosis (CF) is gaining interest. However, phenotypic diversification of bacteria during chronic airway infection presents a potential challenge to this therapy. We recovered and subcultured two or three Burkholderia colonies from each of 12 CF sputum samples. All isolates were tested for their susceptibility to a panel of 65 Burkholderia-targeting phages. We observed that 9 (75%) of the 12 colony sets comprised mixtures of isolates that were sensitive or resistant to one or more of the phages tested. The occurrence of mixed populations of phage-sensitive and phage-resistant Burkholderia in individuals with CF needs to be considered in the development of phage therapy for this patient population.

KW - Bacteriophage therapy

KW - Burkholderia airway infection

KW - Cystic fibrosis

UR - http://www.scopus.com/inward/record.url?scp=105006948080&partnerID=8YFLogxK

U2 - 10.1016/j.jcf.2025.05.008

DO - 10.1016/j.jcf.2025.05.008

M3 - مقالة

C2 - 40451706

SN - 1569-1993

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

ER -

Intra-individual diversity of bacteriophage susceptibility in Burkholderia cultured from cystic fibrosis sputum

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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