Infrared surface plasmon spectroscopy decodes early processes in epithelial host cells upon enteropathogenic Escherichia coli infection

Victor Yashunsky; Benjamin Aroeti

doi:10.1007/978-1-4939-2617-6_20

Infrared surface plasmon spectroscopy decodes early processes in epithelial host cells upon enteropathogenic Escherichia coli infection

Victor Yashunsky, Benjamin Aroeti

Department of Cell and Developmental Biology

The Hebrew University of Jerusalem

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

Original language	American English
Title of host publication	Label-Free Biosensor Methods in Drug Discovery
Publisher	Springer New York
Pages	353-371
Number of pages	19
ISBN (Electronic)	9781493926176
ISBN (Print)	9781493926169
DOIs	https://doi.org/10.1007/978-1-4939-2617-6_20
State	Published - 6 Apr 2015

Keywords

EPEC
Epithelial host cells
Infrared
Spectroscopy
Surface plasmon resonance

All Science Journal Classification (ASJC) codes

General Medicine
General Pharmacology, Toxicology and Pharmaceutics

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10.1007/978-1-4939-2617-6_20

Cite this

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abstract = "Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.",

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T1 - Infrared surface plasmon spectroscopy decodes early processes in epithelial host cells upon enteropathogenic Escherichia coli infection

AU - Yashunsky, Victor

AU - Aroeti, Benjamin

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Y1 - 2015/4/6

N2 - Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

AB - Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

KW - EPEC

KW - Epithelial host cells

KW - Infrared

KW - Spectroscopy

KW - Surface plasmon resonance

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DO - 10.1007/978-1-4939-2617-6_20

M3 - Chapter

SN - 9781493926169

SP - 353

EP - 371

BT - Label-Free Biosensor Methods in Drug Discovery

PB - Springer New York

ER -

Infrared surface plasmon spectroscopy decodes early processes in epithelial host cells upon enteropathogenic Escherichia coli infection

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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