Induction of diffuse axonal brain injury in rats based on rotational acceleration

Dmitry Frank; Israel Melamed; Benjamin F. Gruenbaum; Julia Grinshpun; Ruslan Kuts; Rachel Shvartsur; Abed N. Azab; Mohamad H. Assadi; Max Vinokur; Matthew Boyko

doi:https://doi.org/10.3791/61198

Induction of diffuse axonal brain injury in rats based on rotational acceleration

Dmitry Frank, Israel Melamed, Benjamin F. Gruenbaum, Julia Grinshpun, Ruslan Kuts, Rachel Shvartsur, Abed N. Azab, Mohamad H. Assadi, Max Vinokur, Matthew Boyko

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

Traumatic brain injury (TBI) is a major cause of death and disability. Diffuse axonal injury (DAI) is the predominant mechanism of injury in a large percentage of TBI patients requiring hospitalization. DAI involves widespread axonal damage from shaking, rotation or blast injury, leading to rapid axonal stretch injury and secondary axonal changes that are associated with a long-lasting impact on functional recovery. Historically, experimental models of DAI without focal injury have been difficult to design. Here we validate a simple, reproducible and reliable rodent model of DAI that causes widespread white matter damage without skull fractures or contusions.

Original language	English
Article number	e61198
Journal	Journal of Visualized Experiments
Volume	2020
Issue number	159
DOIs	https://doi.org/10.3791/61198
State	Published - 1 May 2020

Keywords

Diffuse axonal injury (DAI)
Issue 159
Neuroscience
Rat model
Rotational acceleration
Stretch injury
Traumatic brain injury (TBI)
White matter

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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https://doi.org/10.3791/61198

Cite this

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title = "Induction of diffuse axonal brain injury in rats based on rotational acceleration",

abstract = "Traumatic brain injury (TBI) is a major cause of death and disability. Diffuse axonal injury (DAI) is the predominant mechanism of injury in a large percentage of TBI patients requiring hospitalization. DAI involves widespread axonal damage from shaking, rotation or blast injury, leading to rapid axonal stretch injury and secondary axonal changes that are associated with a long-lasting impact on functional recovery. Historically, experimental models of DAI without focal injury have been difficult to design. Here we validate a simple, reproducible and reliable rodent model of DAI that causes widespread white matter damage without skull fractures or contusions.",

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AU - Kuts, Ruslan

AU - Shvartsur, Rachel

AU - Azab, Abed N.

AU - Assadi, Mohamad H.

AU - Vinokur, Max

AU - Boyko, Matthew

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Induction of diffuse axonal brain injury in rats based on rotational acceleration

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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