Inclusion of cross-linked elastin in gelatin/PEG hydrogels favourably influences fibroblast phenotype

Ye Cao, Bae Hoon Lee, Scott Alexander Irvine, Yee Shan Wong, Havazelet Bianco-Peled, Subramanian Venkatraman

Research output: Contribution to journalArticlepeer-review


The capacity of a biomaterial to innatelymodulate cell behaviorwhilemeeting themechanical property requirements of the implant is a much sought-after goal within bioengineering. Here we covalently incorporate soluble elastin into a gelatin-poly (ethylene glycol) (PEG) hydrogel for threedimensional (3D) cell encapsulation to achieve these properties. The inclusion of elastin into a previously optimized gelatin-PEG hydrogel was then evaluated for effects on entrapped fibroblasts, with the aim to assess the hydrogel as an extracellular matrix (ECM)-mimicking 3D microenvironment for cellular guidance. Soluble elastin was incorporated both physically and covalently into novel gelatin/elastin hybrid PEG hydrogels with the aim to harness the cellular interactivity and mechanical tunability of both elastin and gelatin. This design allowed us to assess the benefits of elastin-containing hydrogels in guiding fibroblast activity for evaluation as a potential dermal replacement. It was found that a gelatin-PEG hydrogel with covalently conjugated elastin, supported neonatal fibroblast viability, promoted their proliferation from 7.3% to 13.5% and guided their behavior. The expression of collagen alpha-1(COL1A1) and elastin in gelatin/elastin hybrid gels increased 16-fold and 6-fold compared to control sample at day 9, respectively. Moreover, cells can be loaded into the hydrogel precursor solution, deposited, and the matrix cross-linked without affecting the incorporated cells adversely, thus enabling a potential injectable system for dermal wound healing.

Original languageEnglish
Article number670
Issue number3
StatePublished - 1 Mar 2020


  • Cell encapsulation
  • Dermal substitu
  • Elastin
  • Gelatin
  • Myofibroblast
  • Polyethylene glycol hydrogel

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Polymers and Plastics


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