In vivo targeting of hydrogen peroxide by activatable cell-penetrating peptides

Roy Weinstain; Elamprakash N. Savariar; Csilla N. Felsen; Roger Y. Tsien

doi:10.1021/ja411547j

In vivo targeting of hydrogen peroxide by activatable cell-penetrating peptides

Roy Weinstain, Elamprakash N. Savariar, Csilla N. Felsen, Roger Y. Tsien

Research output: Contribution to journal › Article › peer-review

Abstract

A hydrogen peroxide (H₂O₂)-activated cell-penetrating peptide was developed through incorporation of a boronic acid-containing cleavable linker between polycationic cell-penetrating peptide and polyanionic fragments. Fluorescence labeling of the two ends of the molecule enabled monitoring its reaction with H₂O₂ through release of the highly adhesive cell-penetrating peptide and disruption of fluorescence resonance energy transfer. The H₂O₂ sensor selectively reacts with endogenous H₂O₂ in cell culture to monitor the oxidative burst of promyelocytes and in vivo to image lung inflammation. Targeting H₂O₂ has potential applications in imaging and therapy of diseases related to oxidative stress.

Original language	English
Pages (from-to)	874-877
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	136
Issue number	3
DOIs	https://doi.org/10.1021/ja411547j
State	Published - 22 Jan 2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja411547j

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abstract = "A hydrogen peroxide (H2O2)-activated cell-penetrating peptide was developed through incorporation of a boronic acid-containing cleavable linker between polycationic cell-penetrating peptide and polyanionic fragments. Fluorescence labeling of the two ends of the molecule enabled monitoring its reaction with H2O2 through release of the highly adhesive cell-penetrating peptide and disruption of fluorescence resonance energy transfer. The H2O2 sensor selectively reacts with endogenous H2O2 in cell culture to monitor the oxidative burst of promyelocytes and in vivo to image lung inflammation. Targeting H2O2 has potential applications in imaging and therapy of diseases related to oxidative stress.",

author = "Roy Weinstain and Savariar, {Elamprakash N.} and Felsen, {Csilla N.} and Tsien, {Roger Y.}",

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doi = "10.1021/ja411547j",

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AU - Weinstain, Roy

AU - Savariar, Elamprakash N.

AU - Felsen, Csilla N.

AU - Tsien, Roger Y.

PY - 2014/1/22

Y1 - 2014/1/22

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AB - A hydrogen peroxide (H2O2)-activated cell-penetrating peptide was developed through incorporation of a boronic acid-containing cleavable linker between polycationic cell-penetrating peptide and polyanionic fragments. Fluorescence labeling of the two ends of the molecule enabled monitoring its reaction with H2O2 through release of the highly adhesive cell-penetrating peptide and disruption of fluorescence resonance energy transfer. The H2O2 sensor selectively reacts with endogenous H2O2 in cell culture to monitor the oxidative burst of promyelocytes and in vivo to image lung inflammation. Targeting H2O2 has potential applications in imaging and therapy of diseases related to oxidative stress.

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M3 - مقالة

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EP - 877

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 3

ER -

In vivo targeting of hydrogen peroxide by activatable cell-penetrating peptides

Abstract

All Science Journal Classification (ASJC) codes

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