In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

Devora Gershon; Robert H. Negev-Goldstein; Lama Abd al Razzaq; Shaya Lev; Alexander M. Binshtok

doi:10.1016/j.xpro.2022.101224

In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

Devora Gershon, Robert H. Negev-Goldstein, Lama Abd al Razzaq, Shaya Lev, Alexander M. Binshtok

Department of Medical Neurobiology

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).

Original language	English
Article number	101224
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101224
State	Published - 18 Mar 2022

Keywords

Microscopy
Model Organisms
Neuroscience

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology
General Medicine

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10.1016/j.xpro.2022.101224

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title = "In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals",

abstract = "This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).",

keywords = "Microscopy, Model Organisms, Neuroscience",

author = "Devora Gershon and Negev-Goldstein, \{Robert H.\} and \{Abd al Razzaq\}, Lama and Shaya Lev and Binshtok, \{Alexander M.\}",

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doi = "10.1016/j.xpro.2022.101224",

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AU - Gershon, Devora

AU - Negev-Goldstein, Robert H.

AU - Abd al Razzaq, Lama

AU - Lev, Shaya

AU - Binshtok, Alexander M.

PY - 2022/3/18

Y1 - 2022/3/18

N2 - This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).

AB - This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).

KW - Microscopy

KW - Model Organisms

KW - Neuroscience

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DO - 10.1016/j.xpro.2022.101224

M3 - مقالة

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SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 101224

ER -

In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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