In Vivo Analysis of Intestinal Mononuclear Phagocytes

Caterina Curato, Biana Bernshtein, Tegest Aychek, Steffen Jung

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The study of the intestinal dendritic cell (DC) compartment, its homeostasis, regulation, and response to challenges calls for the investigation within the physiological tissue context comprising the unique anatomic constellation of the epithelial single cell layer and the luminal microbiota, as well as neighboring immune and nonimmune cells. Here we provide protocols we developed that use a combination of conditional cell ablation, conditional compartment mutagenesis, and adoptive precursor transfers to study DC and other intestinal mononuclear phagocytes in in vivo context. We will highlight pitfalls and strengths of these approaches.
Original languageEnglish
Title of host publicationDendritic Cell Protocols
PublisherHumana Press
Chapter18
Pages255-268
Number of pages14
ISBN (Electronic)978-1-4939-3606-9
ISBN (Print)978-1-4939-3604-5
DOIs
StatePublished Online - 4 May 2016

Publication series

NameMethods in Molecular Biology
Volume1423
ISSN (Print)1064-3745

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